王宇,黄江,戴佳琳,廖兴江
WANG Yu1,HUANG Jiang1,DAI Jialin2,LIAO Xingjiang2 (1.Department of Parasitology

• 1:贵阳医学院寄生虫学教研室
• 2:贵阳医学院多媒体形态学实验室

摘要(Abstract)：

目的:分析牛带绦虫成虫谷胱甘肽S-转移酶(GST)基因结构并预测其编码蛋白的结构和功能。方法:利用生物信息学网站如NCBI和ExPASY系统中的生物信息学分析工具,并结合其它分析软件,分析该基因的结构并预测其编码蛋白质的结构和功能。结果:该基因全长908 bp,编码区为135～771 bp,编码212个氨基酸,无各种亚细胞定位序列;与猪带绦虫GST的一致性为93%,相似性为96%;预测3个主要的抗原表位33～53 aa,62～68 aa,179～184 aa位于空间结构上相距较远的分子表面。结论:从牛带绦虫成虫cDNA文库中筛选出GST基因,预测为胞浆型蛋白,可能具有较好的免疫学诊断抗原应用前景。
Objective: To analyze gene structure of glutathione S-transferase(GST) of Taenia saginata,and to predict the structure and function of its encoded protein.Methods: Bioinformatics analysis tools in bioinformatics webs such as NCBI and ExPASY combined with some other analysis softwares were used.Results: The full length of this gene was 908 bp.Its coding region was 135～771 bp,encoding 212 amino acids.The encoded protein didn′t contain any kinds of subcellular localization sequence.Consistency and similarity of the screened gene with that of Taenia solium GST were 93% and 96% respectively.Three major epitopes of GST: 33～53 aa,62～68 aa,179～184 aa were predicted to locate on the surface of GST spatial structure and were far away from each other.Conclusions: GST gene is screened from cDNA library of adult Taenia saginata.GST is predicted to be a cytosolic protein and has good application prospect for immunodiagnosis.

关键词(KeyWords)： 牛肉绦虫;谷胱甘肽S-转移酶;基因;蛋白质序列分析
Taenia saginata;glutathione s-transferase;genes;protein array analysis

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(30760227);; 贵州省科技攻关项目[黔科合NY字(2008)3060]

作者(Author): 王宇,黄江,戴佳琳,廖兴江
WANG Yu1,HUANG Jiang1,DAI Jialin2,LIAO Xingjiang2 (1.Department of Parasitology

DOI: 10.19367/j.cnki.1000-2707.2012.01.003

参考文献(References)：

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