李宏伟,王赟,张春林,龙文涛
LI Hongwei1,WANG Yun2,ZHANG Chunlin3,LONG Wentao3(1.Department of Anatomy

• 1:贵阳医学院人体解剖学教研室
• 2:贵阳医学院医学生物技术教研室
• 3:贵阳医学院生物学教研室

摘要(Abstract)：

目的:获取致倦库蚊(贵阳株)防御素基因CxDefensin全长编码序列。方法:采用RT-PCR方法扩增致倦库蚊(贵阳株)CxDefensin开放阅读框(ORF)序列,并通过生物信息学方法分析其核苷酸和蛋白质序列。结果:CxDefensin ORF序列全长300 bp,编码99个氨基酸,包含1个内含子,蛋白质分子量是10.58 kDa,等电点为6.52。CxDefensin蛋白与其他昆虫的defensin蛋白具有同源性。结论:所获致倦库蚊(贵阳株)防御素基因Cx-Defensin归属昆虫防御素基因家族,为昆虫β型防御素。
Objective: To obtain the full-length coding sequence of defensin gene CxDefensin of Culex quinquefasciatus(Guiyang strain).Methods: Open reading frames of CxDefensin of Culex quinquefasciatus were amplified by RT-PCR,and then bioinformatic methods were used to analyze the nucleotide and protein sequences of CxDefensin.Results: The full-length coding sequence of CxDefensin was 300 bp.CxDefensin encoded 99 amino acids including one intron,and the molecular weight of the protein was 10.58 kDa,isoelectirc point was 6.52.CxDefensin was homology with defensin of other insect.Conclusions: The CxDefensin of Culex quinquefasciatus(Guiyang strain) belongs to defensin gene family of insect,which is one kind of β-defensin.

关键词(KeyWords)： 致倦库蚊;防御素类;序列分析
Culex quinquefasciatus;defensins;sequence analysis

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅基金(黔科合J字(2006)2077号);; 贵州省人才领导小组基金(Q2007-2)

作者(Author): 李宏伟,王赟,张春林,龙文涛
LI Hongwei1,WANG Yun2,ZHANG Chunlin3,LONG Wentao3(1.Department of Anatomy

DOI: 10.19367/j.cnki.1000-2707.2012.04.007

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