张世栋,苑炜,宋林超,黎晨曦,齐磊,庄桂山,于英,卢洪凯
ZHANG Shidong,YUAN Wei,SONG Linchao,LI Chenxi,QI Lei,ZHUANG Guishan,YU Ying,LU Hongkai

• 1:潍坊医学院临床学院泌尿外科
• 2:潍坊市人民医院

摘要(Abstract)：

目的:探讨肥胖在大鼠慢性非细菌性前列腺炎(CAP)性反应中的作用。方法:实验肥胖大鼠模型采用高脂饲料诱导,将诱导成功大鼠随机分为高脂组、普通组和阳性对照组;喂养6周后阳性对照组使用苯甲酸雌二醇按照0.2 mg/kg,每日1次进行皮下注射,连续4周,每周对各组大鼠进行体质量测量并进行标化,并采用前列腺液镜分别对各组前列腺液中卵磷脂小体密度和白细胞进行计数,用ELISA和RT-PCR法对前列腺液中白介素-6(IL-6)和白介素-8(IL-8)炎症因子及其mRNA表达水平进行检测。结果:高脂组6周后体重明显较普通组增加,差异具有统计学意义(P<0.05);高脂组和阳性对照组前列腺液白细胞计数高于普通组,而卵磷脂小体密度较普通组下降,差异有统计学意义(P<0.05);高脂组前列腺液IL-6和IL-8分泌水平均显著高于普通组,IL-6mRNA和IL-8 mRNA相对表达水平显著较普通组上升,差异有统计学意义(P<0.05)。结论:肥胖能够促进前列腺组织IL-6和IL-8的分泌及其mRNA表达对CAP发生具有一定影响。
Objective: To study the relationship between obesity and chronic abacterial prostatitis( CAP) in rats,and provide the basis for the research on the mechanism of obesity in the clinical research of chronic prostatitis. Methods: The experimental obese rats were induced by high fat diet,and the rats were randomly divided into high fat group,normal group and positive control group. After 6 weeks of feeding,the positive control group was injected with estradiol benzoate by 0. 2 mg/kg,once a day for 4 weeks. The rat body wight of each group was measured and standardized,and the density of lecithin bodies and leukocytes in the prostatic fluid were respectively counted by the prostate liquid mirror. By the method of ELISA and RT-PCR interleukin-6( IL-6) in prostatic fluid,interleukin-8( IL-8) cytokines and expression levels of m RNA were detected. Results: High fat group significantly increased in weight than the normal group after 6 weeks,the difference was statistically significant( P< 0. 05). White blood cell count of prostatic fluid of high fat group and positive control group was higher than that of normal group. Lecithin body density was lower than that in normal group,and the difference was statistically significant( P < 0. 05). The secretion levels of prostatic fluid IL-6 and IL-8 in high fat group were significantly higher than those in the normal group. Relative expression level ofIL-6 m RNA and IL-8 m RNA was significantly higher than that of the normal group,the difference was statistically significant( P < 0. 05). Conclusion: Obesity can promote the secretion of prostatic tissue IL-6,IL-8 and their m RNA expression. Therefore,obesity is associated with the occurrence and development of CAP.

关键词(KeyWords)： 肥胖症;模型,动物;前列腺炎;白介素-6;白介素-8
obesity;model,aminal;prostatitis;interluelein 6;interluekin 8

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张世栋,苑炜,宋林超,黎晨曦,齐磊,庄桂山,于英,卢洪凯
ZHANG Shidong,YUAN Wei,SONG Linchao,LI Chenxi,QI Lei,ZHUANG Guishan,YU Ying,LU Hongkai

DOI: 10.19367/j.cnki.1000-2707.2017.10.010

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