康颖倩,刘涛华,曾佳,王梅竹,金方,牟丽丽,吕倩,王颜颜
KANG Yingqian,LIU Taohua,ZENG Jia,WANG Meizhu,JIN Fang,MOU Lili,LV Qian,WANG Yanyan

• 1:贵阳医学院微生物学教研室
• 2:贵阳医学院生物化学与分子生物学教研室

摘要(Abstract)：

目的:对贵州阿哈湖、百花湖及云南滇池不同深度湖水中分离的类蛭弧菌进行分类鉴定,了解云贵地区淡水中类蛭弧菌多样性。方法:培养云贵地区3个湖泊中分离的类蛭弧菌,进行16S rRNA基因测序分析,用MEGA 6.0软件以邻接法构建3个湖泊中类蛭弧菌16S rRNA序列的系统发育树,并以最大似然法(PHYLIP3.1软件)加以验证。结果:从阿哈湖、百花湖及滇池不同深度湖水中分离的类蛭弧菌覆盖率C值为75%,根据类蛭弧菌16S rRNA构建发育树,本研究所得的类蛭弧菌分属3个簇群,γ-Proteobacteria(8.3%)、δ-Proteobacteria(50.0%)及Sphingobacteria(41.7%);在百花湖中分离得到1株路德维希肠杆菌,分离自贵州阿哈湖的6株菌株与噬菌弧菌属的Bacteriovorax sp.EPA同源性较高,分离于滇池的5株菌株的序列为不可培养细菌序列。结论:云贵地区阿哈湖、百花湖及滇池不同深度湖水中的类蛭弧菌具有较高多样性,推测在云南滇池区域存在着尚未开发的新型类蛭弧菌资源。
Objective: To identify Bdellovibrio-like organisms( BALOs) collected from Aha lake and Baihua lake in Guizhou,and from different depths of Dianchi lake in Yunnan,and to study their diversity in fresh water of Yun-Gui region. Method: Sequences of 16 S rRNAs of collected BALOs were submitted to international molecular data bank( GenBank) for similarity,and phylogenetic trees were constructed to analyze the phylogeny with MEGA( version 6. 0). Results: The coverage C value of these BALOs was estimated 75%; The Phylogenetic trees showed that the majority belonged to 3 clusters: γ-Proteobacteria( 8. 3%),δ-Proteobacteria( 50. 0%),and Sphingobacteria( 41. 7%); One strain of Enterobacter ludwigii was isolated from Baihua lake,and other 6 strains from Aha lake showed high homology with Bacteriovorax sp. EPA. However,5 strains from Dianchi lake in Yunnan were failed to be identified at genus level. Conclusions: There is a high diversity of BALOs in Aha lake and Baihua lake in Guizhou,and in different depths of Dianchi lake in Yunnan. It is inferred that there might be new types of BALOA resources in fresh water of Dianchi lake waiting for exploiting.

关键词(KeyWords)： 贵州;云南;水蛭弧菌;基因,rRNA,16S;系统发育树;淡水
Guizhou;Yunnan;Bdellovibrio-like organisms;gene,rRNA,16S;phylogenetic tree;fresh water

Abstract:

Keywords:

基金项目(Foundation): 国家自然基金地方项目(31060006,31260029);; 贵州省社会发展科技攻关项目[黔科合SY字(2011)3017号];; 贵州省卫生厅科技项目(gzwkj2010-1-025);; 贵阳市科技局社会发展与民生计划[筑科合同(2011)10316号]

作者(Author): 康颖倩,刘涛华,曾佳,王梅竹,金方,牟丽丽,吕倩,王颜颜
KANG Yingqian,LIU Taohua,ZENG Jia,WANG Meizhu,JIN Fang,MOU Lili,LV Qian,WANG Yanyan

DOI: 10.19367/j.cnki.1000-2707.2014.05.003

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