李媛媛,张莉莎,肖俊,鲍丽雅,田苗
LI Yuanyuan,ZHANG Lisha,XIAO Jun,BAO Liya,TIAN Miao(Department of Infectious Diseases

• 1:贵阳医学院附院感染科

摘要(Abstract)：

目的:探讨乙型肝炎病毒(HBV)感染者血清标志物模式与HBV DNA水平的关系。方法:采用时间分辨荧光免疫定量分析法(TRFIA)和荧光定量PCR技术(FQ-PCR)分别检测542例乙型肝炎感染者血清HBVM和HBV DNA。结果:542例乙型肝炎感染者血清标本HBVM模式有10种,HBV DNA检出总阳性率83.39%(452/542),各种模式HBV DNA的阳性率存在显著差异性,高拷贝数的HBV DNA主要存在于HBeAg阳性的各种模式中,与其它模式比较有显著差异(P<0.05)。结论:动态监测HBVM和HBV DNA含量有利于对乙肝患者的诊断及疗效提供客观依据。
Objective:To analyze the relationship between modes of serological HBV makers(HBVM) and HBV DNA levels in HBV-infected people.Methods: HBVM and HBV DNA of 542 HBV-infected persons were assayed by using time resolution fluorescence immunological analysis(TRFIA) and fluorescence quantitative PCR(FQ-PCR) techniques.Results: The general HBV DNA positive rate was 83.29%.Ten modes of HBVM were found among the 542 HBV-infected persons,and the positive rates of HBV DNA were significantly different among different modes of HBVM.Higher copy levels of serum HBV DNA occurred mainly in patients with HBeAg positive mode,and were significantly different from those of patients with other modes(P﹤0.05).Conclusions: The dynamic monitoring on contents of HBVM and HBV DNA could provide objective references and is helpful to the diagnosis and treatment of hepatitis B patients.

关键词(KeyWords)： 乙型肝炎病毒;肝炎e抗原,乙型;聚合酶链反应
hepatitis B virus;hepatitis B e antigens;polymerase chain reaction

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 李媛媛,张莉莎,肖俊,鲍丽雅,田苗
LI Yuanyuan,ZHANG Lisha,XIAO Jun,BAO Liya,TIAN Miao(Department of Infectious Diseases

DOI: 10.19367/j.cnki.1000-2707.2009.01.021

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