杨清滔,谷江,石家齐,贾本忠,顾昌世,张永春,朱致晖,姚茂良,刘淼,夏剑锋
YANG Qingtao,GU Jiang,SHI Jiaqi,JIA Benzhong,GU Changshi,ZHANG Yongchun,ZHU Zhihui,YAO Maoliang,LIU Miao,XIA Jianfeng

• 1:贵州医科大学附属白云医院
• 2:贵州医科大学附属医院
• 3:贵州省妇幼保健院

摘要(Abstract)：

目的:研究斯钙素1(stanniocalcin1,STC-1)对肾癌细胞生长的调控机制。方法:体外培养肾癌细胞,用0、0.1、0.5和1.0 nmol/L STC-1溶液浸染肾癌细胞,分别为对照组、低剂量组、中剂量组和高剂量组;分别用MTT法检测各组肾癌细胞的增殖情况,RT-PCR及ELISA法检测各组肾癌细胞中缺氧诱导因子1α(HIF-1α)、STC-1基因及蛋白的表达,荧光分光光度计检测各组细胞内Ca2+水平。结果:随着STC-1蛋白给药浓度的增加,细胞内HIF-1α、STC-1的表达及Ca2+水平逐渐下降,细胞的促增殖作用逐渐降低,在低剂量组出现一过性增加。结论:STC-1蛋白可能通过调节HIF-1α、Ca2+的水平,促进肾癌细胞增殖,而该促增殖作用可能会因为STC-1对HIF-1α的抑制而逐渐减弱,从而调控肾癌细胞的生长平衡。
Objective: To research the regulation machenism of Stanniocalcin1( STC-1) on renal carcinoma cells. Methods: Renal carcinoma cells were cultured in vitro,and STC-1 solutions of different concentration were added to the culture medium. According to the different concentration,renal carcinoma cells were divided into control group,low-dose group( 0. 1 nmol / L STC-1),middledose group( 0. 5 nmol / L STC-1),high-dose group( 1. 0 nmol / L STC-1). The proliferation of cells,expressions of HIF-1α,STC-1 and levels of Ca2 +were detected by MTT,RT-PCR,ELISA and Fluorescence Spectrophotometer respectively. Results: The results showed that the cells treated with STC-1protein exhibited characteristics of proliferation. And along with the increase of STC-1 protein concentration,the proliferation of cells,expressions of HIF-1α and STC-1,levels of Ca2 +were down-regulated. And a transient increase of proliferation of cells occurred in the low-dose group. Conclusions:STC-1 protein may participate in malignant proliferation of renal carcinoma cells through depressing HIF-1α or down-regulation Ca2 +,which could wear off when HIF-1α is inhibited by redundant STC-1,thus regulate the growth balance of renal carcinoma cells.

关键词(KeyWords)： 肾癌细胞;斯钙素1;缺氧诱导因子1α;钙离子;增殖;抑制
renal carcinoma cells;stanniocalcin1;hypoxia-inducible factor 1α;calcium;proliferation;inhibition

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅社会攻关计划项目[黔科合sy字(2011)3060]

作者(Author): 杨清滔,谷江,石家齐,贾本忠,顾昌世,张永春,朱致晖,姚茂良,刘淼,夏剑锋
YANG Qingtao,GU Jiang,SHI Jiaqi,JIA Benzhong,GU Changshi,ZHANG Yongchun,ZHU Zhihui,YAO Maoliang,LIU Miao,XIA Jianfeng

DOI: 10.19367/j.cnki.1000-2707.2015.10.011

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