高秀丽,张敏,蒋蒨,祝君,吴良玉
GAO Xiuli,ZHANG Min,JIANG Qian,ZHU Jun,WU Liangyu(School of Pharmacology

• 1:贵阳医学院药学院
• 2:贵阳医学院药学院
• 3:贵阳医学院药学院
• 4:贵阳医学院药学院

摘要(Abstract)：

目的:建立测定龙血竭滴丸中龙血素B含量的测定方法——高效液相色谱法(HPLC)。方法:采用反相高效液相色谱法,Diamonsil-C18色谱柱,流动相为乙腈-0.4%磷酸溶液(45∶55),检测波长为275 nm,流速为1.0 ml/min,柱温为30℃。结果:龙血素B在8.28~99.36 ng范围内与峰面积呈线性关系(r=0.9996),该制剂中龙血素B的平均回收率为99.03%,RSD为2.09%(n=5)。结论:HPLC法简便,准确,可靠,可用于该龙血竭滴丸的质量控制。
Objective:To establish an HPLC method for determination of loureirin B in Longxuexie drop-pills.Methods: Diamonsil C18 column was used with acetonitrile-0.4% phosphoric acid(45∶55) as the mobile phase,and the detection wavelength was 275 nm.The flow rate was 1.0 ml/min and the temperature of column was 30℃.Results:The calibration curve of loureirin B was linear in the range of 8.28-99.36 ng.The average recovery rate was 99.03%,and RSD was 2.09%(n=5).Conclusion: The method is convenient,precise and reliable for quality control of longxuexie drop-pills.

关键词(KeyWords)： 色谱法,高压液相;龙血竭滴丸;龙血素B
chromatography,high pressure liquid;dragon's blood-pills;loureirin B

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 高秀丽,张敏,蒋蒨,祝君,吴良玉
GAO Xiuli,ZHANG Min,JIANG Qian,ZHU Jun,WU Liangyu(School of Pharmacology

DOI: 10.19367/j.cnki.1000-2707.2007.03.011

参考文献(References)：

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