贵州医科大学学报

2017, v.42;No.198(03) 258-262

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改良6-OHDA注射法建立早期帕金森病大鼠模型
The Establishment of Rat Model of Early Stage Parkinson's Disease by Improved Method with 6-OHDA Injection

韩飞;李成朋;魏操;杨梅;周波;张春林;焦玲;官志忠;禹文峰;
HAN Fei;LI Chengpeng;WEI Cao;Yang Mei;ZHOU Bo;ZHANG Chunlin;JIAO Ling;GUAN Zhizhong;YU Wenfeng;The Key Laboratory of Molecular Biology,Guizhou Medical University;The Education Ministry Key Laboratory of Endemic Diseases and Diseases of Ethenic Minority,Guizhou Medical University;Department of Biology,Guizhou Medical University;Department of Neurology of Affiliated Hospital,Guizhou Medical University;Department of Pathology,Guizhou Medical University;

摘要(Abstract):

目的:改良6-羟基多巴胺(6-OHDA)立体定位注射法建立早期帕金森病(PD)大鼠模型。方法:40只雄性SD大鼠随机分为control组10只,model组30只;于手术前30 min,腹腔注射地昔帕明(25 mg/kg)及帕吉林(5 mg/kg),control组采用立体定位右侧纹状体注射生理盐水,model组注射6-OHDA;术后第3、4周采用圆筒测试及旋转测试测定大鼠的左前肢使用率和旋转速度,免疫组化方法检测黑质内酪氨酸羟化酶(TH)阳性神经元数量。结果:圆筒测试显示madel组大鼠左前肢使用率低于control组(P<0.01),旋转测试显示model组平均向左侧旋转速度(3.48圈/min),明显快于对照组(P<0.01),PD模型成功率为89.66%;免疫组化显示,model组右侧中脑黑质致密部(SNc)部位TH阳性神经元数目较control组右侧减少(44.81±2.89)%,差异有统计学意义(P<0.01),符合临床早期PD诊断标准。结论:改良的6-OHDA注射法建立早期PD大鼠模型简单易行,成功率高。
Objective:To establish rat model of early stage of Parkinson's disease( PD) with improved method of stereotactic injection of 6-OHDA. Methods:Forty male SD rats were randomly divided into control group( group C,10 rats) and model group( group PD,30 rats). Desipramine( 25mg/kg) and pargyline( 5 mg/kg) were injected intraperitoneally 30 minutes before surgery,followed by stereotactic injection of saline( control group) or 6-OHDA( model group) in striatum. Cylinder test and rotation experiments were adopted to evaluate the behavior in 3 and 4 weeks after surgery. Immunohistochemical staining was adopted to analyze the positive cells of tyrosine hydroxylase( TH) in substantia nigra compacta( SNc). Results:Cylinder test showed that the utilization rate of the left forelimb in group PD was lower than that in group C( P < 0. 01). Rotation experiments showed that the average number of turns in group PD was about 3. 48 turns/min while there was no rotation in group C( P < 0. 01). The success rate of PD model was 89. 66%. Immunohistochemistry showed TH positive neurons in right SNc of PD model decreased about 44. 81% compared with that of group C,which was in accordance with the criteria of early stage of PD. Conclusions:The improved method of 6-OHDA injection is a simple,feasible and highly successful approach to establish the model of early PD.

关键词(KeyWords): 6-羟基多巴胺;帕金森病;大鼠;多巴胺神经元;酪氨酸羟化酶
6-Hydroxydopamine;Parkinson's disease;rats;dopamine neurons;tyrosine hydroxylase

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81360199);; 教育部科学技术研究项目(213032A);; 贵州省国际科技合作计划项目[批准号:黔科合外G字(2013)7026号];; 贵州省创新计划项目[黔教合协同创新中心(2014)06];; 贵州省教育厅项目(2015年贵州省普通高等学校地方病和少数民族疾病防控创新团队);; 贵州省科技计划课题任务书[黔科合重大专项字(2014)6008]

作者(Author): 韩飞;李成朋;魏操;杨梅;周波;张春林;焦玲;官志忠;禹文峰;
HAN Fei;LI Chengpeng;WEI Cao;Yang Mei;ZHOU Bo;ZHANG Chunlin;JIAO Ling;GUAN Zhizhong;YU Wenfeng;The Key Laboratory of Molecular Biology,Guizhou Medical University;The Education Ministry Key Laboratory of Endemic Diseases and Diseases of Ethenic Minority,Guizhou Medical University;Department of Biology,Guizhou Medical University;Department of Neurology of Affiliated Hospital,Guizhou Medical University;Department of Pathology,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2017.03.003

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