贵州医科大学学报

2015, v.40;No.176(05) 438-441+446

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R-spondin1在促进人骨髓间充质干细胞成骨分化中的作用
R-spondin1 Promotes Osteogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells

孙博;张弢;杨龙;李靖;任厚相;孙琦;王建吉;叶川;
SUN Bo;ZHANG Tao;YANG Long;LI Jing;REN Houxiang;SUN Qi;WANG Jianji;YE Chuan;Guiyang Medical College;Department of Orthopedics,the 117th Hospital of PLA;

摘要(Abstract):

目的:研究Wnt信号通路激活剂R-spondin1在人骨髓间充质干细胞(h BMSC)成骨分化中的作用。方法:用0、5、10、20μg/L R-spondin1处理h BMSC,利用荧光素酶实验检测R-spondin1对h BMSC中Wnt信号通路的作用,Western blot检测R-spondin1对Wnt信号通路下游靶基因β-catenin蛋白表达的影响;在成骨分化培养基中添加20μg/L R-spondin1诱导h BMSC成骨分化,检测R-spondin1对早期成骨分化指标ALP活性及成骨分化晚期钙沉积、成骨分化标志物OSX、OCN和成骨分化关键转录因子RUNX2表达的影响。结果:R-spondin1增强h BMSC中Wnt信号通路,R-spondin1增强ALP活性和钙沉积,促进OSX、OCN及RUNX2的表达。结论:Rspondin1增强h BMSC中Wnt信号通路的作用,促进h BMSC成骨分化。
Objective: To investigate the effect of R-spondin1,a Wnt signal pathway activator,on osteogenic differentiation of human bone marrow mesenchymal stem cells( h BMSC). Methods:h BMSC were treated with 0,5,10,20 μg / L R-spondin1. Luciferase assay was employed to detect the effect of R-spondin1 on Wnt signal pathway,and Western blot was used to detect the expression of β-catenin which was the downstream target gene of Wnt signal pathway. Alkaline phosphatase( ALP) activity,calcium deposition,and the levels of OSX,OCN,RUNX2 were detected to observe the effect of R-spondin1 on h BMSC osteogenic differentiation. Results: R-spondin1 enhanced Wnt signal pathway,ALP activity,calcium deposition and the expression of OSX,OCN and RUNX2 in h BMSC osteogenic differentiation. Conclusion: R-spondin1 can enhance Wnt signal pathway of h BMSC and promote osteogenic differentiation.

关键词(KeyWords): R-spondin1;Wnt信号通路;人骨髓间充质干细胞;成骨分化;RUNX2
R-spondin1;Wnt signal pathway;Human bone marrow mesenchymal stem cells;Osteogenic differentiation;RUNX2

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基金项目(Foundation): 国家自然科学基金资助项目[81360232];; 贵州省卫生厅资助项目[gzwkj2010-1-042];; 贵州省国际科技合作项目[黔科合外G字2010-7];; 贵州省科技厅社发联合基金项目[黔科合20103166]

作者(Author): 孙博;张弢;杨龙;李靖;任厚相;孙琦;王建吉;叶川;
SUN Bo;ZHANG Tao;YANG Long;LI Jing;REN Houxiang;SUN Qi;WANG Jianji;YE Chuan;Guiyang Medical College;Department of Orthopedics,the 117th Hospital of PLA;

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DOI: 10.19367/j.cnki.1000-2707.2015.05.002

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