陈璐,倪明,王力,潘成云,亢倩,詹雲,赵鹏,王季石
CHEN Lu,NI Ming,WANG Li,PAN Chengyun,KANG Qian,ZHAN Yun,ZHAO Peng,WANG Jishi

• 1:贵州医科大学附属医院血液内科
• 2:贵州医科大学临床医学院
• 3:苏州大学附属第一医院国家血液病临床研究中心

摘要(Abstract)：

目的 探讨粒细胞的髓系来源抑制性细胞(G-MDSCs)和血红素加氧酶-1(HO-1)对异基因造血干细胞移植(allo-HSCT)患者发生急性移植物抗宿主病(aGvHD)的预测价值。方法 收集29例allo-HSCT术患者的一般临床资料，据1974年西雅图分级标准判断aGvHD发生情况分为有aGvHD组和无aGvHD组，抽取2组患者移植后第2、3、4、6、8周及第3月时全血，运用多色流式细胞术(FCM)检测患者外周血G-MDSCs及胞内HO-1和白细胞介素-10(IL-10)的水平，采用表面抗原染色检测G-MDSCs的百分比和绝对值计数，采用破膜染色检测G-MDSCs中HO-1和IL-10的中位荧光强度(MFI)、表达百分比及绝对值计数。结果 2组患者allo-HSCT后第2周于外周血中均可检测到G-MDSCs,但第4周时有aGvHD组患者外周血G-MDSCs较无aGvHD组升高(P<0.05);破膜染色结果显示，有aGvHD组患者于allo-HSCT后第4周时G-MDSCs内HO-1高于无aGvHD组(P<0.05)。结论 allo-HSCT患者术后第4周外周血中G-MDSCs和HO-1的高表达可预测aGvHD的发生。
Objective To explore the predictive value of myeloid derived inhibitory cells(G-MDSCs) and heme oxygenase-1(HO-1) of granulocytes in patients with acute graft versus host disease(aGvHD) who underwent allogeneic hematopoietic stem cell transplantation(allo-HSCT). Methods The general clinical datas of 29 patients with aGvHD who underwent allo-HSCT were collected. According to the Seattle criteria in 1974 and the occurrence of aGvHD, the patients were divided into two groups: aGvHD group and non-aGvHD group. The whole blood samples were collected in the 2~(nd), 3~(rd), 4~(th), 6~(th), 8~(th) weeks and the 3~(rd) months after patients' transplantation. The expression of G-MDSCs and intracellular HO-1 and IL-10 in peripheral blood of the two groups were detected by multi-color flow cytometry(FCM). Concretely, the percentage and absolute count of G-MDSCs were detected by surface antigen staining. In addition, the median fluorescence intensity(MFI), percentage and absolute count of HO-1 and IL-10 in G-MDSCs were detected by membrane-breaking staining. Results G-MDSCs could be detected in peripheral blood of patients in the 2~(nd) week after allo-HSCT. In the 4~(th) week, G-MDSCs in aGvHD group were higher than those in non-aGvHD group(P<0.05). The results of membrane-breaking staining showed that HO-1 in G-MDSCs was higher in aGvHD group than in non-aGvHD group in the 4~(th) week after allo-HSCT(P<0.05). Conclusion The high expressions of G-MDSCs and HO-1 in peripheral blood of the patients in the 4~(th )week after allo-HSCT can predict the occurrence of aGvHD.

关键词(KeyWords)： 血红素加氧酶-1;白细胞介素10;粒细胞的髓系来源抑制性细胞;异基因造血干细胞移植;急性移植物抗宿主病;新鲜外周血
heme oxygenase-1;interleukin-10;granulocytic-myeloid-derived suppressor cells;allogeneic hematopoietic stem cell transplantation;acute graft-versus-host disease;fresh peripheral blood

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(82170168,82160046);; 贵州省科学技术基金项目(黔科合基础-ZK[2021]一般397)

作者(Author): 陈璐,倪明,王力,潘成云,亢倩,詹雲,赵鹏,王季石
CHEN Lu,NI Ming,WANG Li,PAN Chengyun,KANG Qian,ZHAN Yun,ZHAO Peng,WANG Jishi

DOI: 10.19367/j.cnki.2096-8388.2023.06.011

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