陈旭菲,梁逸仙,林春明,蒲育栋,何淑贞
CHEN Xufei,LIANG Yixian,LIN Chunming,PU Yudong,HE Shuzhen

• 1:东莞市第三人民医院产科

摘要(Abstract)：

目的:观察原因不明性复发性流产(URSA)孕妇外周血中自然杀伤T细胞(NKT)、γ-干扰素(IFN-γ)及白细胞介素-4(IL-4)表达水平。方法:30例URSA孕妇为观察组,选择同期同孕龄无流产史孕妇30例作为对照组;两组孕妇于相同孕周(6~14周)抽取空腹静脉血,采用酶联免疫吸附法检测血清NKT、IFN-γ和IL-4水平,流式细胞仪分析外周抗凝血中NKT亚型细胞CD56+CD16-和CD56+CD16+的百分比。结果:观察组孕妇CD56+CD16-细胞百分比明显低于对照组,而细胞毒性较强的CD56+CD16+细胞百分比明显高于对照组(P<0.05);观察组孕妇血清IFN-γ水平和Th1/Th2比值高于对照组,IL-4水平低于对照组(P<0.05)。结论:USRA的发生可能与CD56+CD16+亚型NKT细胞百分比和Th1/Th2比值升高有关。
Objective:To observe NKT,IFN-γ and IL-4 expression level in unexplained recurrent spontaneous abortion.Methods:30 patients with unexplained recurrent spontaneous abortion(URSA)were selected as observation group,and 30 pregnant women with the same gestational age who without history of abortion as control group.After a night of fasting,the levels of NKT,IFN-γ and IL-4 in the two groups were detected by ELISA,and percentage of NKT subtype,cell CD56+CD16-and CD56+CD16+were analyzed by flow cytometry.Results:The CD56+CD16-percentage of observation groups was obviously lower than control group,while CD56+CD16+cells with stronger cytotoxicity showed higher percentage than control group(P < 0.05); the IFN-γ level and Th1/Th2 percentage of observation group were significantly higher than that of the control group,and the level of IL-4 cytokine in observation group was lower than that in control group.Conclusion:The CD56+CD16+NKT subtype cell percentage and increased Th1/Th2 percentage might involve with the USRA.

关键词(KeyWords)： 流产,习惯性;自然杀伤T细胞;γ-干扰素;白细胞介素-4
abortion,habitual;natural killer T cells;interferon gamma;interleukin-4

Abstract:

Keywords:

基金项目(Foundation): 东莞市医疗卫生科技计划一般项目(2016105101038)

作者(Author): 陈旭菲,梁逸仙,林春明,蒲育栋,何淑贞
CHEN Xufei,LIANG Yixian,LIN Chunming,PU Yudong,HE Shuzhen

DOI: 10.19367/j.cnki.1000-2707.2017.02.024

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