张玥,朱晓冉,蔡玥,曹格溪,詹文红
ZHANG Yue,ZHU Xiaoran,CAI Yue,CAO Gexi,ZHAN Wenhong

• 1:河北省人民医院药学部
• 2:河北医科大学药学院

摘要(Abstract)：

目的:研究灯盏花素(Bre)对动脉粥样硬化大鼠氧化应激和炎症反应的影响。方法:选取96只大鼠随机分为正常对照组,模型组,Bre 6、12、24 mg/(kg·d)组和辛伐他汀(SV)组(n=16),后5组采用高脂饲料喂养结合腹腔注射维生素D3的方法建立动脉粥样硬化大鼠模型,各治疗组造模过程同步灌胃给药,正常对照组给予正常饮食及腹腔注射等体积生理盐水;通过HE染色观察主动脉病理变化并进行病变分级;比色法测定血清中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)及过氧化氢酶(CAT)活性和丙二醛(MDA)、活性氧簇(ROS)含量;酶联免疫法测定血浆中C反应蛋白(CRP)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)、白介素-10(IL-10)含量。结果:与模型组比较,Bre干预组大鼠主动脉病理改变呈不同程度改善,Bre24 mg/(kg·d)组病变分级显著降低(P<0.01),Bre 12、24 mg/(kg·d)组血清中SOD、CAT活性显著升高(P<0.05),MDA、ROS含量显著降低(P<0.05),血浆中CRP、TNF-α、IL-1β及IL-6含量显著降低(P<0.05),Bre24 mg/(kg·d)组GSH-Px活性显著升高(P<0.05),IL-10含量显著升高(P<0.01)。结论 :Bre可能通过抑制氧化应激和炎症反应而改善动脉粥样硬化程度。
Objective: To investigate the inhibitory effects of breviscapine( Bre) on oxidative stress and inflammation in atherosclerosis rats. Methods: Eighty rat models were made by dirtying with high fat and injecting VD3 and was divided into 5 groups: model group,Bre 6,12,24 mg/( kg· d) groups and 1. 8 mg/( kg· d) SV group( n = 16),and the drugs were given by intragastric administration at the same time; setting normal control group( n = 16). The histopathological changes of abdominal aorta was observed by HE staining and graded; the activity of antioxidases and the content of MDA,and ROS in serum were determined; the levels of CRP,TNF-α,IL-1 β,IL-6,and IL-10 in the plasma were determined with enzyme linked immunosorbent assay. Results: Compared with model group,the histopathological changes of the rats in Bre treated groups were improved,especially in Bre 24 mg/( kg·d) treated group( P < 0. 01); in Bre 12,24 mg/( kg·d) treated groups,the activity of SOD,CAT in serum was significantly increased( P < 0. 05),the contents of MDA,ROS in serum were significantly decreased( P < 0. 05),and the levels of CRP,TNF-α,IL-1 β,IL-6 in plasma were signifi-cantly decreased( P < 0. 05); in Bre 24 mg/( kg· d) treated group,activity of GSH-Px in serum was significantly increased( P < 0. 05),and the level of IL-10 in plasma was significantly increased( P <0. 01). Conclusion: Bre might mitigate atherosclerosis through restrain oxidative stress and inflammation reaction.

关键词(KeyWords)： 灯盏花素;动脉硬化;氧化性应激;C反应蛋白;肿瘤坏死因子-α;白细胞介素
breviscapine;atherosclerosis;oxidative stress;C-reactive protein;tumor necrosis factor-α;interleukin

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 张玥,朱晓冉,蔡玥,曹格溪,詹文红
ZHANG Yue,ZHU Xiaoran,CAI Yue,CAO Gexi,ZHAN Wenhong

DOI: 10.19367/j.cnki.1000-2707.2017.01.016

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