王世君,黄莉,谢婷婷,夏曙华
WANG Shijun,HUANG Li,XIE Tingting,XIA Shuhua

• 1:贵阳医学院附院临检科

摘要(Abstract)：

目的:检测非小细胞肺癌(NSCLC)组织标本中色素上皮衍生因子(PEDF)、血管内皮生长因子受体(VEGFR-2)的表达,探讨PEDF在NSCLC血管新生中的作用及其与VEGFR-2之间关系。方法:应用免疫组织化学法检测28例NSCLC患者癌组织和远离癌组织的对照组织中PEDF和VEGFR-2的表达;通过标记CD34计数微血管密度(MVD),比较癌组织中PEDF和VEGFR-2阴阳性表达时血管新生情况。结果:癌组织中PEDF和VEGFR-2表达阳性率分别为71.43%、64.29%,与对照组织不表达相比,差异有统计学意义(P<0.05);PEDF和VEGFR-2的共表达率在癌组织和对照组织中分别为39.29%、0%,差异有统计学意义(P<0.01);癌组织MVD(29.30±3.80)低于对照组织(98.86±18.87),差异有统计学意义(P<0.05);癌组织中PEDF阳性表达的MVD显著低于阴性表达的MVD,VEGFR-2阳性表达的MVD显著高于其阴性表达的MVD,差异均有统计学意义(P<0.05)。结论:NSCLC细胞PEDF和VEGFR-2阳性表达百分率高;PEDF阳性表达和VEGFR-2的阴性表达可能与组织MVD有关。
Objective: To investigate the expression of pigment epithelium derived factor( PEDF)and vascular endothelial growth factor receptor( VEGFR-2) in patients with non-small cell lung cancer( NSCLC),the function of PEDF in angiogenesis of non-small cell lung cancer and the relationship with VEGFR-2. Methods: The expressions of PEDF and VEGFR-2 were evaluated by immunohistochemical test in tumor tissues of 28 NSCLC patients and natural tissues. Comparing angiogenesis conditions of positive and negative expressions of PEDF and VEGFR-2 in cancer tissue by marking microvessel density( MVD) of CD34. Results: The positive rates of PEDF and VEGFR-2 were 71. 43% and64. 29% respectively in tumor tissues,PEDF and VEGFR-2 didn't express in control group,there was statistical significance between the cancer group and control group( P < 0. 05); the synchronous expression rate of PEDF and VEGFR-2 in cancer group was markedly higher than that in natural tissue( 39. 29% and 0%),differences were statistically significant( P < 0. 01); MVD( 29. 30 ± 3. 80) in cancer group was lower than that of natural tissue group( 98. 86 ± 18. 87),there was statistical significance( P < 0. 05); MVD in cancer tissue which positively expressed PEDF was remarkably lower than that of cancer tissue which negatively expressed PEDF,MVD in cancer tissue which positively expressed VEGFR-2 was remarkably higher than that of cancer tissue which negatively expressed VEGFR-2( P <0. 05). Conclusions: The positive rate of PEDF and VEGFR-2 were high in NSCLC tissue,and there is a correlation of PEDF positive expression and VEGFR-2 negative expression with tissue MVD.

关键词(KeyWords)： 癌,非小细胞肺;色素上皮衍生因子;血管内皮生长因子受体-2;新生血管化,病理性;免疫组织化学
carcinoma,non-small cell lung;pigment epithelium derived factor;vascular endothelial growth factor receptor 2;neovascularization,pathologic;immunohistochemistry

Abstract:

Keywords:

基金项目(Foundation): 高等学校特色专业建设点[教高函(2010)15];; 贵州省高等学校教改重点项目[黔教高发(2010)208];; 贵阳医学院科研基金(k2006-37)

作者(Author): 王世君,黄莉,谢婷婷,夏曙华
WANG Shijun,HUANG Li,XIE Tingting,XIA Shuhua

DOI: 10.19367/j.cnki.1000-2707.2015.03.006

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