张晓燕,孙文博,梁妍,郝小燕,郭治友,周威
ZHANG Xiaoyan,SUN Wenbo,LIANG Yan,HAO Xiaoyan,GUO Zhiyou,ZHOU Wei

• 1:贵州医科大学基础医学院药学院
• 2:黔南民族师范学院生命科学系

摘要(Abstract)：

目的:建立一种新的反相高效液相色谱法(RP-HPLC-UV)同时测定苦参碱、氧化苦参碱及其在山豆根中的应用。方法:采用RP-HPLC-UV检测方法,Diamonsil C18(250 mm×4.6 mm,5μm)分析色谱柱,流动相为乙腈(A)-25 mmol/L磷酸二氢钾(KH_2PO_4,B)水溶液(10∶90,V/V),检测波长210 nm,流速1.0 m L/min。结果:苦参碱、氧化苦参碱的浓度与峰面积分别在1.00~120.0 mg/L(R=0.999 7)、1.45~144.0 mg/L(R=0.999 9)浓度范围内线性关系良好,平均加样回收率分别为98.69%、102.18%;山豆根药材中苦参碱平均含量0.81 mg/g(生药量),氧化苦参碱平均含量6.98 mg/g(生药量)。结论:该方法简便、准确、实用,适用于同时分析测定苦参碱、氧化苦参碱及其相关药材。
Objective:To establish a new RP-HPLC-UV method for simultaneous determination of matrine and oxymatrine,and to study its application in quality detection of Sophorae Tonkinensis Radix Et Rhizoma. Methods:The RP-HPLC-UV method was chose,the separation was performed on a Diamonsil C18( 250 × 4. 6 mm,5 μm) analytical column( 4. 6 mm × 250 mm,5 μm),and the mobile phase was Acetonitrile-25 mmol/L Potassium dihydrogen phosphate water solution( 10∶ 90,V/V) at a flow rate of 1. 0 m L/min,detection wavelength of 210 nm. Results:The concentrations and peak areas of matrine and oxymatrine were in concentration ranges of 1. 00 ~ 120. 0 μg/m L( R = 0. 999 7) and1. 45 ~ 144. 0 μg/m L( R = 0. 999 9) respectively with good linear relation. Their average sample-added recoveries were 98. 69% and 102. 18% respectively. The average content of matrine in Sophorae Tonkinensis Radix Et Rhizoma was 0. 81 mg/g( crude drug),and that of oxymatrine was 6. 98 mg/g( crude drug). Conclusion:This established method is simple,accurate,and practical,and is suitable for simultaneous determination of matrine,oxymatrine,and other relavant medicinal materials.

关键词(KeyWords)： 苦参碱;氧化苦参碱;山豆根;液相色谱
matrine;oxymatrine;Sophorae Tonkinensis Radix Et Rhizoma;liquid chromatography

Abstract:

Keywords:

基金项目(Foundation): 国家级大学生创新创业训练项目(201410660004);; 贵州省大学生创新创业训练项目(201510660024);; 贵州医科大学博士基金(J-2014-006)

作者(Author): 张晓燕,孙文博,梁妍,郝小燕,郭治友,周威
ZHANG Xiaoyan,SUN Wenbo,LIANG Yan,HAO Xiaoyan,GUO Zhiyou,ZHOU Wei

DOI: 10.19367/j.cnki.1000-2707.2017.03.005

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