贵州医科大学学报

2015, v.40;No.177(06) 552-555

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

氢醌对K562细胞着色性干皮病基因D甲基化的影响
Effects of Hydroquinone on Methylation of XPD Gene in K562 Cells

万秀方;肖芸;郑丹;张越时;龚俊杰;张亚莉;杨国珍;
WAN Xiufang;XIAO Yun;ZHENG Dan;ZHANG Yueshi;GONG Junjie;ZHANG Yali;YANG Guozhen;Department of Medical Laboratory,Guizhou Medical University;

摘要(Abstract):

目的:了解不同剂量苯代谢物氢醌(HQ)对人白血病细胞株K562细胞着色性干皮病基因D(XPD)甲基化水平的影响。方法:分别以终浓度为0、15、30和60μmol/L HQ溶液重复处理K562细胞48 h,采用MTT比色法检测K562细胞增殖能力,采用亚硫酸氢盐处理后测序法检测XPD甲基化水平;观察各组细胞存活率及甲基化率。结果:HQ 0、15、30、60μmol/L处理后,K562细胞存活率分别为(100.00±0.00)%、(85.46±0.60)%、(63.46±7.02)%和(51.20±6.49)%,15μmol/L组与0μmol/L组比较,差异无统计学意义(P>0.05),30μmol/L和60μmol/L组与0μmol/L组比较,差异有统计学意义(P<0.05);XPD基因甲基化水平分别依次为1.03%(3/290)、0.34%(1/290)、0.34%(1/290)和0.70%(2/290),15、30、60μmol/L组与0μmol/L组比较,差异无统计学意义(χ2=1.531,P>0.05)。结论:HQ对K562细胞生长有明显的抑制作用,但对细胞中XPD基因甲基化水平无影响。
Objective: To investigate the effect of different dose of hydroquinone on methylation of xeroderma pigmentosum group D( XPD) in K562 cells. Methods: K562 cells were treated with 0,15,30 and 60 μmol / L of hydroquinone solution for 48 h repeatedly. The inhibition of hydroquinone on K562 cell proliferation was studied by MTT assay. It was first handled by bisulfite then adopting sequencing PCR( BSP) to test methylate level of XPD. Observing the cell survival rate and gene methylation rate. Results: After being treated with 0,15,30 and 60 μmol / L of hydroquinone solution,K562 cells survival rates were( 100. 00 ± 0. 00) %,( 85. 46 ± 0. 60) %,( 63. 46 ± 7. 02) % and( 51. 20 ± 6. 49) % respectively. Comparing 15 μmol/L group and 0 μmol/L group,the differences were not statistically significant( P > 0. 05); comparing 30 μmol / L and 60 μmol / L group,the differences were statistically significant( P < 0. 05). XPD gene methylate levels were: 1. 03%( 3 /290),0. 34%( 1 /290),0. 34%( 1 /290) and 0. 70%( 2 /290). Comparing 15,30,60 μmol / L group and0 μmol / L group,the differences were not statistically significant( χ2= 1. 531,P > 0. 05). Conclusion:Hydroquinone has obvious inhibiting effect on K562 cells growth,but it has no effect on the XPD gene methylate level.

关键词(KeyWords): 氢醌;干皮病,着色性;DNA甲基化;K562细胞;存活率
hydroquinone;xeroderma pigmentosum;DNA methylation;K562 cells;survival rate

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(No.81360437)

作者(Author): 万秀方;肖芸;郑丹;张越时;龚俊杰;张亚莉;杨国珍;
WAN Xiufang;XIAO Yun;ZHENG Dan;ZHANG Yueshi;GONG Junjie;ZHANG Yali;YANG Guozhen;Department of Medical Laboratory,Guizhou Medical University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2015.06.002

参考文献(References):

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享