贵州医科大学学报

2016, v.41;No.189(06) 640-644

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头花蓼对H.pylori感染大鼠模式识别分子DC-SIGN、TLR4表达的影响
Effect of Polygonum Capitatum on Pattern Recognition Molecules DC-SIGN,TLR4

任艳君;莫非;何芸;赵书琴;江明礼;孙朝琴;
REN Yanjun;MO Fei;HE Yun;ZHAO Shuqin;JIANG Mingli;SUN Chaoqin;Department of Clinical Laboratory,Guihzou Medical University;

摘要(Abstract):

目的:观察头花蓼对幽门螺杆菌(H.pylori)感染SD大鼠对免疫应答模式识别受体DC-SIGN、TLR4蛋白和mRNA表达的影响,探讨头花蓼抗H.pylori可能的机制和调节作用。方法:SD大鼠分为空白组、模型组、药物组及药物空白组,模型组及药物组用1×1011cfu/L H.pylori菌液进行灌胃,空白组和药物空白组用脑心浸液肉汤灌胃,8周后,取大鼠胃黏膜显微镜观察组织学改变,鉴定造模是否成功;造模成功后,药物组和药物空白组大鼠取头花蓼灌胃处理,空白组和模型组给予生理盐水灌胃;采用免疫组织化学法和实时荧光定量PCR法(real-time PCR)测定大鼠胃黏膜中DC-SIGN、TLR4蛋白和mRNA表达,观察各组大鼠胃黏不同部位的表达情况。结果:大鼠感染H.pylori后,胃黏膜腺体排列不规则,黏膜下层可见血管充血及大量淋巴细胞浸润,表明造模成功;免疫组织化学和real-time PCR结果显示H.pylori上调了TLR4、DC-SIGN的表达(P<0.05),经头花蓼处理后,表达明显下调(P<0.05);TLR4、DC-SIGN主要在胃黏膜上皮细胞胞浆中表达,H.pylori感染大鼠后TLR4、DC-SIGN表达强阳性。结论:H.pylori感染可上调大鼠胃黏膜上皮细胞DC-SIGN、TLR4的表达,头花蓼处理后,其表达水平降低。
Objective: To observe the effect of Polygonum capitatum on protein and mRNA expression of immune response pattern recognition receptor DC-SIGN,TLR4,and to investigate the possible mechanism and regulation role of Polygonum capitatum anti H. pylori. Methods: SD rats were divided into blank group,model group,drug group and drug blank group. Model group and drug group received intragastric administration of 1 × 1011 cfu / L H. pylori bacteria solution while blank group and drug blank group received intragastric administration of brain heart infusion broth. 8 weeks later,the microscope was used to observe the histological changes of gastric mucosa of rats in order to identify whether the model was successful to. After modeling was successful,drug group and drug blank group were treated with Polygonum capitatum by intragastric administration while blank group and model group received physiological saline by intragastric administration. Immunohistochemistry and real-time PCR were adopted to detect the protein and mRNA expression of in gastric mucosa of rats. The expression of different parts of gastric mucosa of rats in each group was observed. Results: After rats were infected with H. pylori,the gastric mucosal glands showed irregular arrangement,with visible vascular congestion and a large number of lymphocytes infiltration in the submucosal layer,which proved that model was established successfully. The results of Immunohistochemistry and real-time PCR showed that H. pylori up-regulated TLR4,DC-SIGN expression level( P < 0. 05). After the treatment of Polygonum capitatum,TLR4 and DC-SIGN expression level significantly down-regulated( P < 0. 05).TLR4,DC-SIGN were mainly expressed in cytoplasm of gastric mucosal epithelial cells. After rats were infected with H. pylori,TLR4,DC-SIGN showed strongly positive expression. Conclusion: H. pylori infection can up-regulate DC-SIGN and TLR4 expression level in gastric mucosa epithelial cells,but after treatment of Polygonum capitatum their expression levels significantly decrease.

关键词(KeyWords): 螺杆菌,幽门;基因表达;免疫;Toll样受体;C型凝集素受体;头花蓼
Helicobacter pylori;gene expression;immune;Toll like receptor;type C lectin receptor;Polygonum capitatum

Abstract:

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基金项目(Foundation): 高等学校特色专业建设点[No(2010)15];; 贵州省科技计划课题[黔科合J字(20142027)号];贵阳市科技计划项目[筑科合同(20141001)号];; 贵州省科技合作计划基金[黔科合LH(2015)7417号];; 贵州省卫生计生委科学技术基金[gzwjkj2015-1-003]

作者(Author): 任艳君;莫非;何芸;赵书琴;江明礼;孙朝琴;
REN Yanjun;MO Fei;HE Yun;ZHAO Shuqin;JIANG Mingli;SUN Chaoqin;Department of Clinical Laboratory,Guihzou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2016.06.005

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