屈璐,候胜开,高楠楠,潘艳伶
QU Lu,HOU Shengkai,GAO Nannan,PAN Yanling

• 1:贵州医科大学
• 2:贵州医科大学附院

摘要(Abstract)：

目的:建立2型糖尿病周围神经病变(DPN)大鼠模型。方法:SD大鼠40只分为对照组(10只)和模型组(30只),模型组予高脂高糖饲料喂养及2~3次小剂量腹腔注射1%的链脲佐菌素(STZ)建立糖尿病(DM)动物模型,对照组给予普通饲料喂养并注射同等剂量生理盐水,模型组中DPN造模成功的作为DPN组,DPN造模不成功但DM造模成功的作为DM组;于实验第14周后,检测大鼠坐骨神经动作电位传导速度、血清神经生长因子(NGF)水平及坐骨神经病理形态学变化。结果:30只大鼠中DM成模24只,其中15只大鼠并发DPN,DPN成模率62.5%;与对照组相比,DPN组大鼠坐骨神经动作电位传导速度、血清NGF含量均明显降低(P<0.05);与DM组相比,DPN组大鼠血清NGF含量明显降低(P<0.05)。结论:高脂高糖饮食联合腹腔注射STZ可成功制作DPN大鼠模型,其机制与大鼠血清NGF水平降低有关。
Objective: To establish Type II diabetic peripheral neuropathy model of rats. Methods:40 SD rats were divided into normal control group( Group A,10) and model group( Group B,30);Group B fed with high fat and sugar diet. Giving Group B 2 to 3 times of small dose of intraperitoneal injection of 1 % STZ concentration and use self-made mold to form the diabetes model. Group A fed with normal diet and equal amount of saline water. Group B DPN model was established successfully and then used as DPN group; DPN modeling failed ones but DM modeling succeed ones were used as DM group. 14 weeks later,rats' sciatic potential conduction velocity,NGF and sciatic pathomorphology was tested. Results: Out of 30 rats,24 succeeded in DM modeling,15 rats were complicated by DPN,DPN modeling success rate was 62. 5 %; comparing with control group,rats ' sciatic potential conduction velocity and NGF of DPN group decreased significantly( P < 0. 05); comparing with DM group,NGF content of DPN group decreased significantly( P < 0. 05). Conclusion: The method can successfully induce the establishment of type 2 diabetic peripheral neuropathy model,the mechanism is correlated with rats' serum NGF level decrease.

关键词(KeyWords)： 糖尿病,非胰岛素依赖型;周围神经病;坐骨神经;模型,动物;大鼠,Sprague-Dawley
diabetes mellitus,non-insulin dependent;peripheral neuropathy;sciatic nerve;model,animal;rats,Sprague-Dawley

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金项目[黔科合J字(2009)2192号]

作者(Author): 屈璐,候胜开,高楠楠,潘艳伶
QU Lu,HOU Shengkai,GAO Nannan,PAN Yanling

DOI: 10.19367/j.cnki.1000-2707.2017.01.013

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