梁燕,王磊,雷鸣,顾佳,郑明,张芍,李素艳,陈让,肖云,严志凌
LIANG Yan,WANG Lei,LEI Ming,GU Jia,ZHENG Ming,ZHANG Shao,LI Suyan,CHEN Rang,XIAO Yun,YAN Zhiling

• 1:云南开放大学健康护理学院
• 2:昆明医科大学第三附属医院妇科
• 3:昆明医科大学第三附属医院重症医学科
• 4:元谋县人民医院妇产科

摘要(Abstract)：

目的 探讨Kirsten大鼠肉瘤病毒癌基因同源物(KRAS)基因中的单核苷酸多态性位点(SNPs)与云南汉族人群宫颈癌发生风险的相关性。方法 选取云南地区汉族人群宫颈癌患者496例作为病例组、健康个体499例作为对照组，采用TaqMan探针基因分型法对KRAS基因3′-UTR区域3个SNPs位点rs12587(G>T)、rs12245(A>C)、rs1137282(A>G)进行基因分型，并分析其与云南汉族地区人群宫颈癌易感性的相关性。结果 KRAS基因3′-UTR区域3个SNPs位点的等位基因和基因型在病例组和对照组中分布频率比较，差异无统计学意义(P>0.05);单倍型分析结果显示，单倍型rs12587T-rs12245A-rs1137282A在病例组中的分布频率显著低于对照组(P<0.001),该单倍型可能是宫颈癌发生的保护性因素(OR=0.517, 95%CI:0.384～0.697)。结论 KRAS基因3′-UTR区域SNP位点rs12587(G>T)、rs12245(A>C)及rs1137282(A>G)组成的单倍型可能与云南汉族人群宫颈癌发生的风险相关。
Objective To investigate the correlation between single nucleotide polymorphisms(SNPs) in Kirsten rat sarcoma viral oncogene homolog(KRAS) gene and the risk of cervical cancer in Yunnan Han population. Methods A total of 496 cervical cancer patients as case group and 499 healthy controls of Han ethnic group in Yunnan were enrolled in this study. TaqMan probe genotyping method was used to detect three single nucleotide polymorphism(SNP) in KRAS gene rs12587(G>T), rs12245(A>C), rs1137282(A>G), and the correlation between genotype and its susceptibility of cervical cancer in Yunnan Han population was analyzed. Results There was no significant difference in the distribution frequencies of alleles and genotypes of the three SNP loci in the 3'-UTR region of the KRAS gene between case group and control group(P>0.05). Halotype analysis indicated that the halotype frequency of rs12587T-rs12245A-rs1137282A in case group was significantly lower than that of control group(P<0.001). Such halotype could be the protective factor of occurrence of cervical cancer(OR=0.517, 95%CI was 0.384-0.697). Conclusion The SNP loci rs12587(G>T), rs12245(A>C), and rs1137282(A>G) in the 3'-UTR region of KRAS gene might be associated with the risk of cervical cancer in Yunnan Han population.

关键词(KeyWords)： 宫颈癌;KRAS基因;SNPs;相关性;云南;汉族
cervical cancer;KRAS gene;single nucleotide polymorphisms(SNPs);correlation;Yunnan;han population

Abstract:

Keywords:

基金项目(Foundation): 云南省教育厅科学研究基金项目(2022J0602);; 云南省科技厅-昆明医科大学应用基础研究联合专项基础研究计划项目(202201AY070001-139)

作者(Author): 梁燕,王磊,雷鸣,顾佳,郑明,张芍,李素艳,陈让,肖云,严志凌
LIANG Yan,WANG Lei,LEI Ming,GU Jia,ZHENG Ming,ZHANG Shao,LI Suyan,CHEN Rang,XIAO Yun,YAN Zhiling

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