潘玲,何常
PAN Lin,HE Chang

• 1:贵州医科大学病理科
• 2:贵州医科大学附院病理科

摘要(Abstract)：

目的:探讨髓样细胞白血病-1基因(Mcl-1)及染色质重塑基因A(ARIDIA)蛋白在卵巢上皮癌组织中的表达。方法:应用免疫组化Envision法检测113例卵巢上皮癌(Ⅰ期42例、Ⅱ期28例、Ⅲ期30例及Ⅳ期13例)组织、40例卵巢囊腺瘤组织及20例正常卵巢组织中的Mcl-1、ARIDIA蛋白表达,比较2种蛋白表达阳性率在3种组织和不同分期卵巢上皮癌组织的表达情况。结果:卵巢上皮癌组织中Mcl-1蛋白表达阳性率明显高于卵巢囊腺瘤和正常组织,而ARIDIA明显低于卵巢囊腺瘤和正常组织(P<0.05);Mcl-1蛋白在卵巢上皮性癌Ⅰ期、Ⅱ期、Ⅲ期及Ⅳ期的组织中表达阳性率逐渐升高,ARIDIA蛋白表达阳性率逐渐降低,差异有统计学意义(P<0.05)。结论:卵巢上皮性癌的发生、发展与Mcl-1蛋白表达升高和ARIDIA蛋白表达降低有关。
Objective: To investigate expression of myeloid cell leukemia-1(Mcl-1)and chromatin remodeling gene A( ARIDIA) protein in epithelial ovarian carcinomas tissue. Methods: Adopting IHC Envision assay to test Mcl-1 and ARIDIA protein expression of 113 epithelial ovarian carcinomas tissue(42 cases of Phase I,28 cases of Phase Ii,30 cases of Phase III and 13 cases of Phase IV),40 ovarian cystadenoma tissue and 20 healthy ovarian tissue; comparing expression of positive rate of 2 protein expression in 3 different tissues and phases ovarian carcinomas tissues. Results: Mcl-1 protein expression positive rate of ovarian carcinomas tissue was obviously higher than that of epithelial ovarian carcinomas and healthy tissue( P < 0. 05);expression positive rate of Mcl-1 protein in epithelial ovarian carcinomas Phase I,II,III and IV increased gradually,ARIDIA protein expression positive rate decreased gradually,difference was statistically significant( P < 0. 05). Conclusion: Occurrence of epithelial ovarian carcinomas and development was correlated with Mcl-1 protein expression increase and ARIDIA protein expression decrease.

关键词(KeyWords)： 细胞凋亡;抑癌基因;髓样细胞白血病-1蛋白;染色质重塑基因A蛋白;卵巢肿瘤;免疫组织化学
apoptosis;tumor suppressor gene;myeloid cell leukemia-1 protein;chromatin remodeling gene A protein;ovarian tumor;immunohistochemistry

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅基金(E2010-4)

作者(Author): 潘玲,何常
PAN Lin,HE Chang

DOI: 10.19367/j.cnki.1000-2707.2016.02.008

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