赵淦,李盈甫,马千里,陈楠,谭芳,周紫云,赵雨笛,陈珺,刘楠楠,梁燕
ZHAO Gan,LI Yingfu,MA Qianli,CHEN Nan,TAN Fang,ZHOU Ziyun,ZHAO Yudi,CHEN Jun,LIU Nannan,LIANG Yan

• 1:云南大学生命科学学院
• 2:昆明医科大学第一附属医院
• 3:昆明医科大学第三附属医院
• 4:中国医学科学院&北京协和医学院医学生物学研究所

摘要(Abstract)：

目的:研究IL21基因中单核苷酸多态性(SNPs)位点rs2221903、rs12508721和rs907715与云南汉族人群非小细胞肺癌易感性的关系。方法:采用Taq Man探针基因分型的方法对437例非小细胞肺癌患者(病例组)和602例健康对照人群(对照组)的IL21基因SNPs位点rs2221903、rs12508721和rs907715进行基因分型,并分析其等位基因和基因型在病例组和对照组中的分布特征;根据连锁不平衡结果构建单倍型,并分析各单倍型在病例组和对照组中分布频率。结果:rs2221903位点等位基因C在病例组中的分布频率高于对照组(P=0. 016),该位点等位基因C可能会增加云南汉族人群非小细胞肺癌的患病风险(OR=1. 41,95%CI为1. 07～1. 85);单倍型结果分析显示单倍型rs2221903C-rs12508721C-rs907715C可能增加非小细胞肺癌的患病风险(OR=1. 42,95%CI为1. 07～1. 87),单倍型rs2221903T-rs12508721C-rs907715C可能与非小细胞肺癌患病风险降低有关(OR=0. 58,95%CI为0. 39～0. 85);而rs12508721和rs907715位点等位基因和基因型在对照组和病例组的分布频率的差异无统计学意义(P> 0. 05);结论:IL21基因中多态性位点rs2221903位点等位基因C可能会使云南汉族人群非小细胞肺癌患病风险增加。
Objective: To investigate the relationship between single nucleotide polymorphisms(SNPs) rs2221903,rs12508721 and rs907715 in IL21 gene with the occurrence and development of non-small cell lung cancer(NSCLC) in a Chinese Han group. Methods: TaqMan assay was used for genotyping the 3 SNPs in IL21 gene in 437 non-small cell lung cancer patients(case group) and 602 healthy individuals(control group). The allelic and genotypic distributions of the SNPs in the two groups were analyzed. Linkage disequilibrium result was used to construct haplotype,then analyzing haplotype distribution frequency in case group and control group. Results: The results showed that the allelic frequency of rs2221903 C was significantly higher in case group than control group(P = 0.016),and this indicated that the C allele might be the risk factor of NSCLC(OR = 1. 41; 95% CI: 1.07 ～ 1. 85). In addition,the haplotypic analysis showed that the haplotype rs2221903 C-rs12508721 Crs907715 C might be a risk factor(OR = 1. 42; 95% CI: 1. 07 ～ 1. 87) for NSCLC,and rs2221903 Trs12508721 C-rs907715 C might be a protective factor(OR = 0. 58; 95% CI: 0. 39 ～ 0. 85). Allelic and genotype rs12508721 and rs907715 distribution frequency in both groups showed no statistical significance(P > 0. 05). Conclusion: Rs2221903 in IL21 might be associated with NSCLC in a Chinese Han group in Yunnan province.

关键词(KeyWords)： 白细胞介素21;基因;肺肿瘤;多态性,单核苷酸;云南;汉族
interleukin 21;gene;lung neoplasms;polymorphisms,single nucleotide;Yunnan;Han population

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81573206);; 云南省应用基础研究计划-昆医联合专项资助项目(2017FE468-193);; 云南省医疗卫生单位内设研究机构科研项目(2016NS107)

作者(Author): 赵淦,李盈甫,马千里,陈楠,谭芳,周紫云,赵雨笛,陈珺,刘楠楠,梁燕
ZHAO Gan,LI Yingfu,MA Qianli,CHEN Nan,TAN Fang,ZHOU Ziyun,ZHAO Yudi,CHEN Jun,LIU Nannan,LIANG Yan

DOI: 10.19367/j.cnki.1000-2707.2018.10.020

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