万晓龙,王加中,董蕾,史海涛,杨龙宝
WAN Xiaolong,WANG Jiazhong,DONG Lei,SHI Haitao,YANG Longbao

• 1:西安交通大学第二附属医院消化内科

摘要(Abstract)：

目的:探讨锌指蛋白217(ZNF217)在胰腺癌组织中的表达,分析其与胰腺癌患者临床特征的关系。方法:收集61例胰腺癌患者术后切除的胰腺癌组织及癌旁组织标本,各61例;采用免疫组化法检测组织标本中ZNF217蛋白表达,分析ZNF217蛋白表达与胰腺癌患者临床病理特征(性别、年龄、瘤体直径、TNM分期、组织学分级及淋巴结转移)的关系。结果:免疫组织化学染色结果显示,胰腺癌组织ZNF217蛋白阳性着色强度大于癌旁组织,阳性细胞比例高于癌旁组织,胰腺癌组织ZNF217蛋白阳性率及免疫组织化学评分显著高于癌旁组织,差异有统计学意义(P <0. 01);分析发现,胰腺癌男性与女性患者、年龄<50岁与年龄≥50岁患者、组织学分级G1~G2级与G3级患者间ZNF217蛋白阳性表达率比较,差异无统计学意义(P> 0. 05);而瘤体直径≥2 cm、TNM分期Ⅲ~Ⅳ期及淋巴结转移患者ZNF217蛋白阳性表达率分别显著高于瘤体直径<2 cm、TNM分期Ⅰ~Ⅱ期及无淋巴结转移患者,差异有统计学意义(P <0. 05、或P <0. 01)。结论:胰腺癌组织ZNF217蛋白阳性表达率显著高于癌旁组织,且ZNF217蛋白阳性表达与瘤体直径增大、高TNM分期及淋巴结转移有关。
Objective: To explore the expression of Zinc finger protein 217(ZNF217) in pancreatic cancer tissue and its relationship with the clinical characteristics. Methods: 61 cases pancreatic cancer patients who were treated with surgical removal in our hospital Mar. 2016 to Dec. 2017 were selected,the pancreatic cancer tissue specimens and corresponding para-carcinoma tissue specimens were collected. The expression of ZNF217 protein in pancreatic cancer and para-carcinoma tissue specimens were detected by immunohistochemical method; the relationship between the expression of ZNF217 protein and clinicopathological characteristics(such as gender,age,tumor diameter,TNM stage,histological grade and lymph node metastasis) of patients with pancreatic cancer were analyzed. Results:Immunohistochemistry staining showed that the positive staining intensity of ZNF217 protein was greater than that of paracancerous tissue,and the proportion of positive cells was higher than that of paracancerous tissue. The ZNF217 protein positive rates in pancreatic cancer and para-carcinoma tissue were72. 13%,16. 39% respectively,compared with para-carcinoma tissue,the positive rate and immunohistochemical score of ZNF217 were significantly higher than that of paracancerous tissue,and the difference was statistically significant(P < 0. 01). Pearson χ2 test showed that comparison of positive expression rate of ZNF217 between male and female patients with pancreatic cancer,patients with age< 50 and age > 50,histological grade G1 ~ G2 and G3 had no significant difference(P > 0. 05);while the positive expression rate of ZNF217 protein in patients with tumour diameter ≥2 cm,TNM stage Ⅲ stage-Ⅳ stage and lymphatic metastasis were higher significantly than that in patients with tumour diameter < 2 cm,TNM stageⅠstage-Ⅱstage and without lymphatic metastasis,the difference was statistically significant(P < 0. 05 or P < 0. 01). Conclusion: The positive expression rate of ZNF217 protein in pancreatic cancer tissue is higher than that in para-carcinoma tissue,and the positive expression of ZNF217 protein has positive correlation with tumor diameter increasing,high TNM stages and lymphatic metastasis.

关键词(KeyWords)： 胰腺肿瘤;锌指蛋白217;基因表达;免疫组织化学染色
pancreatic cancer;ZNF217 protein;gene expression;immunohistochemical staining

Abstract:

Keywords:

基金项目(Foundation): 陕西省科学技术厅重点研发项目(2018SF-042)

作者(Author): 万晓龙,王加中,董蕾,史海涛,杨龙宝
WAN Xiaolong,WANG Jiazhong,DONG Lei,SHI Haitao,YANG Longbao

DOI: 10.19367/j.cnki.1000-2707.2018.11.023

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