石静,马洪,段晓峰
SHI Jing,MA Hong,DUAN Xiaofeng

• 1:贵州医科大学

摘要(Abstract)：

目的:研究细胞周期蛋白依赖性激酶1(CDK1)在口腔鳞状细胞癌(OSCC)组织中的表达及与OSCC患者临床特征之间的关系。方法:选择20例正常口腔黏膜组织(正常对照组)以及64例OSCC组织(OSCC组),用免疫组织化学方法检测CDK1蛋白的表达,采用Real-Time q PCR检测CDK1 mRNA的表达,比较OSCC组及正常对照组CDK1蛋白及mRNA的表达水平,同时观察不同分化程度、不同临床分期及是否发生淋巴结转移患者的CDK1 mRNA的表达水平。结果:OSCC组CDK1蛋白阳性表达率显著高于正常对照组(P<0.01); OSCC组CDK1 mRNA表达明显高于正常对照组(P<0.01);中、低分化OSCC组织中CDK1 mRNA相对表达量显著高于高分化OSCC组织(P<0.05),Ⅲ、Ⅳ期OSCC组织中CDK1 mRNA相对表达量也高于Ⅰ、Ⅱ期OCSS组织(P<0.05);有淋巴结转移OCSS组织中CDK1 mRNA相对表达量也高于无淋巴结转移组织(P<0.05)。结论:CDK1表达增高可能在OSCC发生及发展过程中起到重要的作用。
Objective:To investigate the expression of CDK1 in OSCC(oral squamous carcinoma)and its relationships relationshpi with clinical features of OSCC patients.Methods:Using Real-Time qPCR to detect the expression of CDK1 mRNA in 20 cases of normal oral mucosa tissues(normal control group) and 64 cases of OSCC tissues(OSCC group) and immunohistochemical assay to detect CDK1 protein expression.Comparing expression level of CDK1 protein and mRNA of both groups.Meanwhile,observing CDK1 mRNA expression level of patients with degrees of differentiation,different clinical staging and lymph nodes metastasis or not.Results:The results showed that the protein positive expressions rate of CDK1 in OSCC group was significantly higher than that of normal control group(P<0.01).The expression of CDK1 mRNA in OSCC was significantly higher than that of the normal control group(P<0.01).The relative expression of CDK1 mRNA in low and medim differentiated OSCC tissue was significantly higher than that of high differentiated OSCC tissues(P<0.05);CDK1 mRNA relative expression in stage Ⅲ and IV OSCC tissues was also higher than that in stage I and II(P<0.05);lymph node metastasis OCSS group showed higher CDK1 m ENA relative expression than none metastasis OCSS group(P<0.05).Conclusion:The increased expression of CDK1 may play an important role in the pathogenesis and development of OSCC.

关键词(KeyWords)： 细胞周期蛋白依赖性激酶1;口腔肿瘤;癌,鳞状细胞;实时荧光定量聚合酶链反应;免疫组织化学
Cyclin dependent kinase 1;oral neoplasms;carcinoma,squamous cell;reverse-transcription polymerase chain reaction;immunohistochemical assay

Abstract:

Keywords:

基金项目(Foundation): 贵州省国际合作计划项目[黔科合外G字(2014)7009号]

作者(Author): 石静,马洪,段晓峰
SHI Jing,MA Hong,DUAN Xiaofeng

DOI: 10.19367/j.cnki.1000-2707.2019.01.012

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