林晓坚,袁兆伟,潘彩燕,孙黔云,宛蕾
LIN Xiaojian,YUAN Zhaowei,PAN Caiyan,SUN Qianyun,WAN Lei

• 1:贵州医科大学基础医学院药理学教研室
• 2:贵州省中国科学院天然产物化学重点实验室

摘要(Abstract)：

目的:了解红景天苷(Sal)对小鼠体外血栓的影响及作用机制。方法:制备小鼠洗涤血小板,分为空白对照组、Sal(12.5、25.0、50.0、100.00及200.0μmol/L)5个剂量组和裂解液裂解组,通过乳酸脱氢酶(LDH)法检测Sal对血小板的毒性作用;将洗涤血小板分为空白对照组、模型组、阳性药组(替罗非班0.5 mg/L)、Sal低剂量组(25μmol/L)及Sal高剂量组(50μmol/L),以凝血酶诱导血块回缩,观察各组血块回缩情况,同时采用酶联免疫吸附方法测定血栓素B_2(TXB_2)含量。结果:裂解液裂解组小鼠洗涤血小板LDH活性明显高于空白对照组,Sal各剂量组小鼠洗涤血小板LDH活性分别明显低于裂解液裂解组(P<0.01);模型组小鼠洗涤血小板中凝血酶能引起明显的血块回缩、血块比率明显低于空白对照组(P<0.01),替罗非班组则受到显著抑制、血块比率明显大于模型组(P<0.01),Sal低剂量和高剂量组均可抑制凝血酶诱导的血块回缩、血块比率大于模型组(P<0.05或P<0.01);模型组洗涤血小板TXB_2含量水平明显高于空白对照组,Sal低、高剂量组和阳性对照组小鼠低于模型组(P<0.05或P<0.01)。结论:Sal对血小板无明显毒性,可以明显抑制凝血酶诱导的血块回缩,此过程可能是通过减少血小板TXA_2的含量发挥作用。
Objective: To study the effect of Salidroside(Sal) on thrombus in vitro and explore its possible antithrombotic mechanism. Methods: The washed platelets were divided into 7 groups, namely, the blank control group, five dosages' groups of Sal(12.5 μmol/L, 25.0 μmol/L, 50.0 μmol/L, 100.0 μmol/L, 200.0 μmol/L) and Lysate lysis group. The toxicity of Sal to platelets was detected by lactate dehydrogenase(LDH) method. The platelets were incubated with normal Sal(25 μmol/L, 50 μmol/L) in advance, and the clot retraction was induced by thrombin to observe the effect of Sal on the clot retraction. Enzyme linked immunosorbent assay was used to determine the content and level of thromboxane B_2(TXB_2) in mice induced by thrombin after incubating Sal(25 μmol/L, 50 μmol/L).Results: Compared with the control group, there was no significant difference in the release of lactic dehydrogenase(LDH) after the treatment of different doses of Sal on platelets(P>0.05). Compared with the control group, preincubated Sal platelets showed significantly inhibited clot retraction(P<0.05 or P<0.01).The TXB2 content of Sal treated platelets was significantly lower than that of the model group(P<0.05).Conclusion: Salidroside has no obvious toxicity to platelets, and can significantly inhibit thrombin induced clot retraction and reduce the expression of TXA_2.

关键词(KeyWords)： 血小板;乳酸脱氢酶类;血栓素B_2;红景天苷;血块回缩
platelet;lactate dehydrogenase;thromboxane B_2;salidroside;clot retraction

Abstract:

Keywords:

基金项目(Foundation): 贵州省创新人才团队建设项目[黔科合平台人才(2016)5625]

作者(Author): 林晓坚,袁兆伟,潘彩燕,孙黔云,宛蕾
LIN Xiaojian,YUAN Zhaowei,PAN Caiyan,SUN Qianyun,WAN Lei

DOI: 10.19367/j.cnki.1000-2707.2019.12.008

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