白芨多糖对大鼠血小板聚集、凝血功能及TXB_2、6-keto-PGF_(1α)表达的影响Effect of Bletilla striata Polysaccharides on Rat Platelet Aggregation,Coagulation Function,TXB_2 and 6-keto-PGF_(1α) Expression
董莉;董永喜;刘星星;廖尚高;王爱民;李勇军;
DONG Li;DONG Yongxi;LIU Xingxing;LIAO Shanggao;WANG Aimin;LI Yongjun;Guizhou Provincial Key Laboratory of Pharmaceutics,Guiyang Medical University;Engineering Research Center of Education Ministry for the Development and Application of Ethnic Medicine and TCM,Guiyang Medical University;
摘要(Abstract):
目的:观察白芨多糖对大鼠血小板聚集、凝血功能及血浆血栓素B2(TXB2)、6-酮-前列腺素F1α(6-keto-PGF1α)水平的影响,探讨白芨多糖的止血机制。方法:健康SD大鼠60只,随机分为正常对照组(生理盐水)、白芨多糖高剂量组(12 g/kg)、白芨多糖中剂量组(6 g/kg)、白芨多糖低剂量组(3 g/kg)、云南白药组(0.4g/kg),连续给药14 d;股动脉采血,测定各组大鼠血小板聚集率、凝血酶原时间(PT)、凝血酶时间(TT)、部分凝血活酶时间(APTT)、纤维蛋白原(FIB)、TXB2和6-keto-PGF1α水平。结果:与空白对照组比较,白芨多糖中、高剂量能增加大鼠血小板聚集率,缩短PT、APTT和TT,显著增加FIB、TXB2含量和降低6-keto-PGF1α含量(P<0.05或P<0.01)。结论:白芨多糖可能通过激活内源性,外源性凝血系统,促进血小板聚集和调节TXB2、6-keto-PGF1α的水平,发挥止血功能。
Objective: To investigate the hemostatic mechanism of Bletilla striata(Thunb.) Reichb.F.(BSP) Polysaccharides on rat platelet aggregation,coagulation function,thromboxane B2(TXB2)and 6-keto-prostaglandin-F1α(6-keto-PGF1α)expression. Methods: Adult SD rats(n =60) were randomly divided into control group( normal saline),high dose Bletilla striata Polysaccharides group(12 g/kg),medium dose Bletilla striata Polysaccharides group(6 g/kg),low dose Bletilla striata Polysaccharides group(3 g /kg) and Yunnan Baiyao group(0. 4 g /kg),continuous infusion for 14 days;Culling femoral blood to test rats ' platelet aggregation rate,prothrombin time( PT),thrombin time(TT),activated partial thromboplastin time(APTT),fibrinogen(FIB),bTXB2 and 6-keto-PGF1α. Results: Comparing to control group,the group of BSP medium dose and high dose could improve platelet aggregation,shorten PT,APTT and TT,increase the level of FIB and TXB2,and decrease the level of 6-keto-PGF1α(P <0.05,P <0.01).Conclusion: The hemostatic effect of BSP relates to activate intrinsic and extrinsic pathways to promote platelet aggregation and modulate the level of TXB2 and 6-keto-PGF1α.
关键词(KeyWords):
白芨多糖;血小板聚集;凝血功能;血栓素;前列腺素
polysaccharides from Bletilla striata;platelet aggregation;coagulation function;thromboxane;prostraglandins
基金项目(Foundation): 国家自然科学基金(NO.81360636);; 贵州省中药现代化专项项目[黔科合中药字(2010)5027号;黔科合重G字(2013)4001];; 贵阳市科学技术计划项目[筑科合同(2012204)20号]
作者(Authors):
董莉;董永喜;刘星星;廖尚高;王爱民;李勇军;
DONG Li;DONG Yongxi;LIU Xingxing;LIAO Shanggao;WANG Aimin;LI Yongjun;Guizhou Provincial Key Laboratory of Pharmaceutics,Guiyang Medical University;Engineering Research Center of Education Ministry for the Development and Application of Ethnic Medicine and TCM,Guiyang Medical University;
DOI: 10.19367/j.cnki.1000-2707.2014.04.004
参考文献(References):
- [1]李伟平,何良艳,丁志山.白芨的应用及资源现状[J].中华中医药学刊,2012(1):158-160.
- [2]宋立人.现代中药学大辞典(上册)[M].北京:人民卫生出版社,2001:367.
- [3]国家药典委员会.中国药典(2010版1部)[M].北京:人民卫生出版社,2010:158.
- [4]国家中医药管理局《中华本草》编委会.中华本草-苗药卷[M].贵阳:贵州科技出版社,2005:197-199.
- [5]任华忠,何毓敏,杨丽.白芨化学成分其药理活性研究进展[J].亚太传统医药,2009(2):134-140.
- [6]王军,王春宏,任晓玲,等.白芨胶的临床应用[J].中国医院药学杂志,2004(9):556.
- [7]张卫明.植物资源开发研究与应用[M].南京:东南大学出版社,2005:284.
- [8]林焕阳,金大地,陈建民,等.云南白药对颈椎管扩大成形术出血量影响的研究[J].中国中医现代远程教育,2008(6):1171-1172.
- [9]叶剑锋,严伟民,甘卓慧,等.云南白药对大鼠血小板聚集及膜糖蛋白表达的影响[J].中国现代应用药学杂志,2004(2):100-103.
- [10]唐正龙,王兴,伊彪,等.云南白药对血小板的释放与代谢反应的影响[J].贵阳医学院学报,2009(3):282-289.
- [11]侯小涛,慕丽群,周江煜,等.伊血安颗粒的主要药效学实验研究[J].中国实验方剂学杂志,2010(1):108-109.
- [12]庞爱明,阮长耿.绿茶的主要成分表没食子儿茶素没食子酸酯(EGCG)对血小板功能影响的研究[J].中国血液流变学杂志,2004(1):37-39.
- [13]刘茜,宋睿璞,陈桂敏.柏叶汤中凉血止血药对脾胃虚寒出血大鼠血清TXB2,6-keto-PGF1α的影响[J].中国实验方剂学杂志,2012(5):172-174.
- [14]王珍,杨靖亚,宋书杰,等.三七素对凝血功能的影晌及止血机制[J].中国新药杂志,2014(3):356-359.
文章评论(Comment):
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- 白芨多糖
- 血小板聚集
- 凝血功能
- 血栓素
- 前列腺素
polysaccharides from Bletilla striata - platelet aggregation
- coagulation function
- thromboxane
- prostraglandins
- 董莉
- 董永喜
- 刘星星
- 廖尚高
- 王爱民
- 李勇军
DONG Li- DONG Yongxi
- LIU Xingxing
- LIAO Shanggao
- WANG Aimin
- LI Yongjun
- Guizhou Provincial Key Laboratory of Pharmaceutics
- Guiyang Medical University
- Engineering Research Center of Education Ministry for the Development and Application of Ethnic Medicine and TCM
- Guiyang Medical University
- 董莉
- 董永喜
- 刘星星
- 廖尚高
- 王爱民
- 李勇军
DONG Li- DONG Yongxi
- LIU Xingxing
- LIAO Shanggao
- WANG Aimin
- LI Yongjun
- Guizhou Provincial Key Laboratory of Pharmaceutics
- Guiyang Medical University
- Engineering Research Center of Education Ministry for the Development and Application of Ethnic Medicine and TCM
- Guiyang Medical University