毛久凤,夏茜,吴镭,杨红,周成菊,方艺,董强
MAO Jiufeng,XIA Qian,WU Lei,YANG Hong,ZHOU Chengju,FANG yi,DONG qiang

• 1:贵州医科大学口腔医学院口腔修复学教研室
• 2:贵州医科大学
• 3:贵州医科大学附属口腔医院口腔内科
• 4:贵阳市口腔医院

摘要(Abstract)：

目的:探讨不同表面处理方法对钛表面的成骨细胞膜片碱性磷酸酶(ALP)活性的影响。方法:原代培养大鼠成骨细胞,通过形态学及ALP鉴定成骨细胞;以机械抛光处理的钛片为对照组,以棕刚玉颗粒喷砂材料处理的钛片为喷砂酸蚀(SLA)组,分别构建成骨细胞膜片,膜片连续培养1周或2周时,测量成骨细胞膜片中反映成骨效应的ALP活性的改变。结果:原代培养细胞ALP阳性,经形态及特性鉴定为成骨细胞;连续培养1周或2周时,SLA组ALP活性均高于对照组,差异有统计学意义(P<0.05);各组膜片ALP活性第1周高于第2周,差异有统计学意义(P<0.05)。结论:钛表面性质能够影响成骨细胞膜片的成骨分化能力。
Objective: To study the ontogenesis of osteoblasts cell sheets with different treated surface of titanium. Methods: Primary cultured rat osteoblasts were identified through morphology observation,alkaline phosphatase staining. Mechanically polished titanium sheets served as control group.The cell sheets were treated with brown fused alumina material as SLA group. Constructing osteoblast cell sheets respectively,and cultured successively for 1 or 2 weeks. Then,measuring changes of ALP activity. Results: Primary cultured cell ALP was positive,the morphology of cells conformed to the characteristics of osteoblasts. The alkaline phosphatase activity of the SLA group was higher than that of control group when cultured successively for 1 or 2 weeks,differences were statistically significant( P < 0. 05). The alkaline phosphatase activity of the cell sheets formed in 1stweek was higher than that of 2ndweek,differences were statistically significant( P < 0. 05). Conclusion: The surface properties of titanium could affect the osteogenic capacity of cell sheet.

关键词(KeyWords)： 细胞膜片;钛;表面处理;碱性磷酸酶;细胞培养;大鼠,Sprague Dawley
cell shee;titanium;surface treatment;alkaline phosphatase;cell culture;rats,Sprague-Dawley

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅省校合作协议项目[黔科合LH字(2014)7115];; 贵阳市科技计划项目[筑科合同(2014)100139]

作者(Author): 毛久凤,夏茜,吴镭,杨红,周成菊,方艺,董强
MAO Jiufeng,XIA Qian,WU Lei,YANG Hong,ZHOU Chengju,FANG yi,DONG qiang

DOI: 10.19367/j.cnki.1000-2707.2016.04.013

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