王颜颜,欧维正,夏茂宁,黄劲,秦万,蒙俊,王明栋,明春艳,康颖倩
WANG Yanyan,OU Weizheng,XIA Maoning,HUANG Jing,QIN Wan,MENG Jun,WANG Mingdong,MING Chunyan,KANG Yingqian

• 1:贵州医科大学微生物学教研室
• 2:贵州医科大学
• 3:贵阳市公共卫生救治中心
• 4:贵州医科大学生化与分子生物学教研室

摘要(Abstract)：

目的:了解2012-2013年贵阳市非结核分枝杆菌(NTM)的流行情况。方法:对贵阳市某医院2012-2013年就诊的可疑结核病患者进行分枝杆菌的培养鉴定,采用PCR-荧光探针及基因芯片法进行鉴定,并对相关结果进行分析。结果:1 500例患者经分离培养和PCR-荧光探针及基因芯片法鉴定为结核分枝杆菌感染的1 469例,鉴定为NTM感染的31例,占2.06%,其中8株为鸟分枝杆菌,10株为胞内分枝杆菌,9株为龟或脓肿分枝杆菌,偶然分枝杆菌、瘰疬分枝杆菌、堪萨斯分枝杆菌及蟾蜍分枝杆菌各1株,分别占NTM 25.80%、32.25%、29.03%、3.23%、3.23%、3.23%、3.23%;NTM感染的阳性患者中,男女比例为1.21∶1,30~59岁年龄段占61.29%;不同性别的感染高峰年龄段不同,男性以75~89岁、女性则以45~59岁年龄段。结论:贵阳市存在一定比例的NTM菌感染,以鸟分枝杆菌、胞内分枝杆菌、龟/脓肿分枝杆菌为主。
Objective:To investigate the prevalence of nontuberculosis mycobacterium( NTM) in Guiyang city from 2012 to 2013. Methods:NTM culturing and strain identification were adopted in patients with suspected tuberculosis in a hospital of Guiyang during the period from 2012 to 2013,PCR-fluorescent probe and gene chip were adopted for identification and analyzing relevant results.Results:1469 strains were identified as Mycobacterium tuberculosis from 1500 strains of clinical isolated and PCR-fluorescent probe and gene chip method,31 strains were NTM,accounted for 2. 06%.Of which 8 strains were Mycobacterium avium,10 strains were Mycobacterium intracellulare,9 strains were Turtle/Mycobacterium abscessus; while there was only one Mycobacterium fortuitum,Mycobacterium scrofulaceum,Kansas Mycobacterium tuberculosis and Mycobacterium xenopi respectively. The proportion was 25. 80%,32. 25%,29. 03%,3. 23%,3. 23%,3. 23%,3. 23% in NTM. Among the NTM-positive patients,the proportion of male to female was 1. 21:1. Patients at age of 30 ~ 59 was61. 29%. Affection peak age group was different in different genders:male was between 75 to 89 and female was between 45 to 59. Conclusions:There is a certain proportion of NTM affection in Guiyang.Mycobacterium avium,Mycobacterium intracellulare and Turtle/Mycobacterium abscessus were the main pathogenic bacteria.

关键词(KeyWords)： 分枝杆菌,结核;分枝杆菌,鸟;分枝杆菌,非结核;菌种鉴定;基因芯片
Mycobacterium tuberculosis;Mycobacterium avium;nontuberculosis Mycobacterium;strain identification;gene chip

Abstract:

Keywords:

基金项目(Foundation): 国家自然基金地方项目(31260029);; 贵州省国际科技合作项目(黔科合外G字[2014]7006);; 贵阳市人民政府-贵州医科大学科学技术联合基金“贵阳市科技局创新团队”项目(GY2015-18);; 贵州省科技计划项目[黔科合(2010)3154号];; 贵阳市科技计划项目[筑科合同(2014001)33号]

作者(Author): 王颜颜,欧维正,夏茂宁,黄劲,秦万,蒙俊,王明栋,明春艳,康颖倩
WANG Yanyan,OU Weizheng,XIA Maoning,HUANG Jing,QIN Wan,MENG Jun,WANG Mingdong,MING Chunyan,KANG Yingqian

DOI: 10.19367/j.cnki.1000-2707.2017.03.017

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