贵州医科大学学报

2018, v.43;No.219(12) 1402-1406

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miR200c及EZH2在乳腺癌中的表达及关系
Expression and Significance of miR200c and EZH2 in Breast Cancer

张雪云;
ZHANG Xueyun;Breast and Thyroid Surgery,Affiliated Hospital of Qinghai University;

摘要(Abstract):

目的:探究miR200c及组蛋白甲基化转移酶2(EZH2)在乳腺癌中的表达及关系。方法:收集70例浸润性导管乳腺癌(IDC)患者的乳房上皮标本(10例导管原位癌、20例原发性IDC、20例区域淋巴结受累原发性IDC及20例远处转移IDC)及15例正常乳房上皮临床组织标本,同时收集转染si AFAP-EZH2质粒(si EZH2组)、转染siRNA control(control组)及不进行转染(空白组)的乳腺癌MDA-MB-468细胞;采用实时定量PCR检测临床组织标本及各组乳腺癌细胞中miR-200c及EZH2 mRNA表达水平,比较EZH2 mRNA高表达及低表达乳腺癌组织中miR-200c表达水平;观察下调EZH2表达对乳腺癌细胞中miR-200c mRNA表达的影响,Transwell实验检测下调EZH2表达对乳腺癌细胞侵袭能力的影响。结果:与对照组比较,各组乳腺癌组织标本中miR-200c表达水平显著升高(P <0. 05),且淋巴结受累及远处转移严重程度越高,miR-200c水平升高越显著(P <0. 05);各组乳腺癌组织标本中EZH2 mRNA表达显著降低(P <0. 05),乳腺癌有远处转移组EZH2 mRNA表达水平显著低于淋巴结受累组(P <0. 05);与control组和空白组比较,si EZH2组EZH2 mRNA表达水平显著降低(P <0. 05); EZH2高表达组乳腺癌组织中miR-200c表达水平较EZH2低表达组明显降低(P <0. 05);转染si AFAPEZH2后的乳腺癌细胞miR-200c表达水平明显高于control组和空白组(P <0. 05); Transwell实验结果显示,下调EZH2表达时MDA-MB-468细胞的侵袭能力增加(P <0. 05)。结论:乳腺癌的发生发展与EZH2及miR-200c表达有关,调控乳腺癌细胞EZH2及miR-200c表达的可调控乳腺癌的侵袭转移。
Objective: To investigate the expression and significance of miR200 c and EZH2 in breast cancer. Methods: A total of 70 IDC( invasive ductal carcinoma) and 15 normal breast epithelium were collected,including 10 cases of ductal carcinoma,20 primary IDC,20 primary lymph nodes were involved in primary IDC and 20 patients were distantly metastasis,and 15 normal breast epithelium as control group. Collecting and transfecting si AFAP-EZH2 plasmid( si AFAP-EZH2 group),transfecting siRNA control( control group) and no transfection MDA-MB-468 cell( Blank group). Adopting real time PCR to detect clinical tissue samples and miR-200 c and EZH2 mRNA expression level of breast cancer cells in all groups; comparing miR-200 c mRNA expression level in high expression of EZH2 mRNA and low expression of breast cancer tissues. Observing effect of down regulated EZH2 expression on miR-200 c mRNA in breast cancer cells; Transwell experiment detected down regulated EZH2 expression on breast cancer invasive capacity. Results: The expression of miR-200 c in patients' group was significantly higher than that in the control group( P < 0. 05),and increased with the involvement of lymph nodes and distant metastasis severity( P < 0. 05); the expression of EZH2 mRNA in patients' groups was significantly lower( P < 0. 05); the expression of EZH2 mRNA in group metastasis was significantly lower than that in group lymph nodes( P < 0. 05). Compared with control group and blank group,EZH2 mRNA expression level of si EZH2 group was obviously lowered( P < 0. 05); the expression level of miR-200 c in EZH2 high expression group was significantly lowered than that in EZH2 low expression group P < 0. 05). After breast cancer cells were transfected with si AFAP-EZH2,miR-200 c expression level was significantly higher than that in control group and blank group( P < 0. 05). Transwell experiment indicated that,when EZH2 was down-regulated,the MDA-MB-468 cells invasive capacity was significantly decreased( P < 0. 05). Conclusion: The expression of miR-200c/EZH2 can regulate the invasion and metastasis of breast cancer,and miR-200 c and EZH2 is expected to become a molecular marker for the invasion and metastasis of breast cancer.

关键词(KeyWords): 组蛋白甲基化转移酶2;微小RNA;miR-200c;乳腺肿瘤;肿瘤转移;聚合酶链式反应;基因表达
histone methylation transferase 2;microRNA;miR-200c;breast neoplasms;tumor metastasis;polymerase chain reaction;gene expression

Abstract:

Keywords:

基金项目(Foundation): 青海大学附属医院中青年科研基金资助项目(ASRF-2015-YB-09)

作者(Author): 张雪云;
ZHANG Xueyun;Breast and Thyroid Surgery,Affiliated Hospital of Qinghai University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2018.12.008

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