叶丹,冯红超,宋宇峰,马竹君
YE Dan,FENG Hongchao,SONG Yufeng,MA Zhujun

• 1:贵州医科大学
• 2:贵阳市口腔医院口腔颌面外科
• 3:贵州省食品药品监督管理局

摘要(Abstract)：

目的:研究信号传导与转录激活因子3(STAT3)及肿瘤相关巨噬细胞(TAMs)与人口腔鳞状细胞癌发生、浸润、转移的关系及机制。方法:采用免疫组化的方法检测42例人口腔鳞状细胞癌和15例正常口腔粘膜组织中STAT3、CD68+(巨噬细胞标记物)、CD163+(M2型巨噬细胞标记物)的表达及浸润数量并探讨3者与临床病理指标之间的关系。结果:口腔鳞癌中STAT3的表达,CD68+、CD163+细胞的浸润数量与正常组比较明显高于正常组(P<0.05);癌组织中STAT3的阳性表达与分化、临床分期、有无淋巴结转移有关(P<0.05),但与患者年龄、性别无关; STAT3的表达与CD163+细胞浸润数呈正相关(r=0.397,P<0.05),CD68+和CD163+细胞在中、低分化中的浸润数量明显高于高分化组(P<0.05); CD68+与CD163+之间呈正相关(r=0.329,P=0.034)。结论:STAT3表达可能与肿瘤恶性程度相关,其高表达可能使巨噬细胞更多向M2形转化,而M2型巨噬细胞可能促进OSCC的浸润转移。
Objective:To investigate the Correlation between STAT3 expression and tumor associated macrophage content in oral squamous cell carcinoma.Methods:The expression and infiltration of STAT3,CD68+(Macrophage marker) and CD163+(M2 macrophage marker) in 42 cases of human oral squamous cell carcinoma and 15 cases of normal oral mucosa were detected by immunohistochemical method.Results:The expression of STAT3,the number of CD68+and CD163+cells in oral squamous cell carcinoma were significantly higher than those in normal group(P<0.05).The positive expression of STAT3 was related to differentiation,clinical stage and lymph node metastasis(P<0.05),but not related to age and sex.The expression of STAT3 had positive correlation with the number of CD163+cell infiltration(r=0.397,P<0.05).The infiltration of CD68+and CD163+cells in the middle and low differentiation group was significantly higher than that in the highly differentiated group(P<0.05).CD68+had positive correlation with CD163+(r=0.329,P=0.034).Conclusion:The expression of STAT3 may be related to the degree of malignancy of the tumor,and its high expression may lead to more M2-shaped transformation of macrophages,while M2 macrophages may promote the infiltration and metastasis of OSCC.

关键词(KeyWords)： 口腔鳞状细胞癌;转录激活因子3;肿瘤相关巨噬细胞;M2型巨噬细胞
oral squamous cell carcinoma;transcription activation 3;tumor associated macrophages;M2 macrophage

Abstract:

Keywords:

基金项目(Foundation): 贵阳市卫生局科学技术计划项目[筑卫科技合同(2016)026号];; 贵阳市科技计划项目[筑科合同(2018)1-56号]

作者(Author): 叶丹,冯红超,宋宇峰,马竹君
YE Dan,FENG Hongchao,SONG Yufeng,MA Zhujun

DOI: 10.19367/j.cnki.1000-2707.2019.01.009

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