贵州医科大学学报

2017, v.42;No.198(03) 360-364

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循环miRNA-296-5p与原发性高血压发病机制的相关性研究
Preliminary Study on the Mechanism of miRNA-296-5p in Essential Hypertension

唐春仕;谭利辉;卢新林;陈文江;
TANG Chunshi;TAN Lihui;LU Xinlin;CHEN Wenjiang;Cardiovascular Department,the Fourth People's Hospital of Chenzhou;Emergency Department,the Fourth People's Hospital of Chenzhou;Cardiovascular Department,the Affiliated Hospital of Guangdong Medical University;

摘要(Abstract):

目的:探讨血浆中miRNA-296-5p与原发性高血压(EH)的相关性。方法:选取EH患者80例和非EH患者80例,收集两组患者基本病史、心血管疾病用药情况等一般资料,采用qRT-PCR检测其血浆中循环miRNA-296-5p的表达水平,生物信息学预测miRNA-296-5p的靶基因以及其调控的信号通路,ELISA检测两组患者血浆中TGF-β1和ACE2的表达,并分析两者与miRNA-296-5p的相关性。结果:EH组与非EH组在年龄、性别、BMI、基本病史和部分血液学生化指标比较,差异无统计学意义(P>0.05);EH组血浆中循环miRNA-296-5p表达水平明显高于非EH(P<0.01);ROC曲线分析发现曲缺中miRNA-296-5p曲线下面积为0.844 5,95%可信区间为(CI)0.782 5~0.906 4(P<0.01),灵敏度80.00%,特异性90.00%;生物信息学预测发现miRNA-296-5p的靶基因可能为血管转化生长因子-β1(TGF-β1)和血管紧张素转化酶2(ACE2),可能调控血管转化生长因子/SMAD(TGF-β/SMAD)、磷脂酰肌醇-3激酶(PI3K-Akt)、胰岛素/胰岛素生长因子信号通路(Insulin/IGF)、丝裂原活化蛋白激酶(MAPK)和NOTCH等信号通路,并可能参与多种circRNA的调控;ELISA检测发现EH组TGF-β1和ACE2的表达明显高于非EH组(P<0.01),miRNA-296-5与TGF-β1、ACE2均呈正相关(r=0.317 6、0.417 1,P<0.01)。结论:血浆中循环miRNA-296-5p可能通过调控TGF-β1和ACE2导致EH的发生,并可以作为EH新的临床诊断标志物。
Objective:To identify the correlation of plasma miRNA-296-5p with essential hypertension( EH). Methods:80 EH cases and 80 non-EH cases with their general information such as case history and medication on cardiovascular disease,then adopting qRT-PCR to test expression level of circulating miRNA-296-5p,and its biological information was predicted. Then the test carried out ELISA to detect the probable target gene of miRNA-296-5p. Results:There was no statistical difference in age,gender,BMI,basic history and basic laboratory tests between EH group and non-EH group( P > 0. 05); the circulating miRNA-296-5p expression level in EH group was significantly higher than non-EH group( P < 0. 01); ROC curve analysis found that the area under the curve was0. 844 5,and the 95% CI was 0. 782 5-0. 906 4( P < 0. 01); bioinformatics prediction found thatTGF-β1 and ACE2 might be the target gene of miRNA-296-5p,and might regulate TGF-β/SMAD,PI3K-Akt,Insulin/IGF,MAPK and NOTCH signaling pathways together with regulating multiple circRNA.ELISA revealed that TGF-β1 and ACE2 expression levels were significantly higher in EH cases(P<0.01);miRNA-296-5 was positively correlated with TGF-β1 and ACE2(r=0.317 6,0.4 171,P<0.01).Conclusion:Circulating miRNA-296-5p can be a biomarker of EH,and may participate in the mechanism of EH by regulating TGF-β1 and ACE2.

关键词(KeyWords): 血管转化生长因子β1;血管紧张素转化酶2;高血压;生物标志物;微小RNA-296-5p
transforming growth factor-beta1;angiotensin conrerting enzyme 2;hypertension;biomarkers;circRNA-296-5p

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作者(Author): 唐春仕;谭利辉;卢新林;陈文江;
TANG Chunshi;TAN Lihui;LU Xinlin;CHEN Wenjiang;Cardiovascular Department,the Fourth People's Hospital of Chenzhou;Emergency Department,the Fourth People's Hospital of Chenzhou;Cardiovascular Department,the Affiliated Hospital of Guangdong Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2017.03.026

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