吴林菁,姜丰,苏菊,吴朝花,张旭,张嫩玲,沈祥春,陶玲
WU Linjing,JIANG Feng,SU Ju,WU Chaohua,Zhang Xu,ZHANG Nenling,SHEN Xiangchun,TAO Ling

• 1:贵州医科大学药学院
• 2:天然药物资源优效利用重点实验室
• 3:贵州省高等学校天然药物药理与成药性评价特色重点实验室

摘要(Abstract)：

目的:优选艳山姜挥发油的最佳提取工艺,分析其挥发油化学成分。方法:以挥发油提取率为指标,以浸泡时间、料液比、提取时间为考察因素进行正交试验,确定艳山姜挥发油的提取工艺条件;采用气质联用仪(GC-MS)考察不同提取时间对挥发油化学成分的影响。结果:正交试验和方差分析结果表明,最佳提取工艺条件为浸泡1 h,料液比1∶12,提取时间7 h;从1、5及7 h提取时间的挥发油中分离出了107个化合物,鉴定了70个化合物,有部分成分含量差异大;所鉴定的化合物相对含量占各自挥发油总量的92.909%、95.001%及87.020%。结论:在优选的提取工艺条件下,挥发油提取率较高;艳山姜不同提取时间挥发油的成分种类无变化,但部分成分含量差异大。
Objective: To optimize extraction process of volatile oil from Alpiniae Zerumbet and analyze its chemical compositions. Method: With the volatile oil extraction rate as the index and the soaking time,solid-liquid ratio,and extraction time as factors,the orthogonal test was conducted to determine the extraction process conditions of volatile oil from Alpiniae Zerumbet; GC-MS was adopted to investigate the effect of different extraction time on chemical compositions of Alpiniae Zerumbet volatile oil. Results: Orthogonal test and variance analysis results showed that the optimum extraction condition was: soaking for 1 h,solid-liquid ratio of 1∶ 12,extraction time 7 h. There were 107 compounds separated from Alpiniae Zerumbet volatile oil from which 70 compounds were identified when the extraction time was in the range from 1. 5 and 7 h. The relative content of identified compounds from the volatile oil accounted for 92. 909% of the total,95. 001% and 87. 020%,respectively. Conclusion: Inthe optimal extraction condition,the extraction rate of the volatile oil is higher. There is no change of components and varieties at different extraction time for Alpiniae Zerumbet,but the content of some components is tremendously different.

关键词(KeyWords)： 色谱法,气相;植物提取物;艳山姜;挥发油;成分分析;提取工艺
gas chromatography-mass spectrometry;plant extracts;Alpiniae Zerumbet;volatile oil;component analysis;extraction process

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技合作项目[黔科合LH字(2016)7368];贵州省科技合作项目[黔科合LH字(2015)7339];; 贵州省大学生创新创业训练计划项目(201610660031);; 贵州省教育厅科技拔尖人才支持项目[黔教合字KY字(2016)073];; 贵州省高等教育科技创新团队[黔教合人才团队字(2014)31];; 贵州省科技创新团队[黔科合人才团队(2015)4025];; 贵州省高层次创新型人才百层次人才[贵州省科技厅黔科合人才(2015)4029];; 贵州省科技厅项目[黔科合J字(2011)2290]

作者(Author): 吴林菁,姜丰,苏菊,吴朝花,张旭,张嫩玲,沈祥春,陶玲
WU Linjing,JIANG Feng,SU Ju,WU Chaohua,Zhang Xu,ZHANG Nenling,SHEN Xiangchun,TAO Ling

DOI: 10.19367/j.cnki.1000-2707.2017.06.008

参考文献(References)：

• [1]贵州省药品监督管理局.贵州省中药材、民族药材质量标准[S].贵州:贵州科技出版社,2003:187.
• [2]陈训,黄龙华,龙成昌,等.艳山姜(土砂仁)与几种“砂仁”的鉴别[J].贵州科学,2004(4):53-55.
• [3]沈祥春,胡函帅,肖海涛.GC-MS法分析艳山姜根茎、茎、叶及果实等部位挥发油化学成分[J].药物分析杂志,2010(8):1399-1403.
• [4]陶玲,沈祥春,彭佼,等.艳山姜全果及不同部位挥发油化学成分GC-MS分析[J].中成药,2009(6):909-911.
• [5]Tao L,Hu HS,Shen XC.Endothelium-dependent vasodilatation effects of the essential oil from Fructus Alpiniae Zerumbet(EOFAZ)on rat thoracic aortic rings in vitro[J].Phytomedicine,2013(5):387-393
• [6]文波,令狐克刚,徐旖旎,等.艳山姜挥发油抑制JNK1/2/3磷酸化对ox-LDL诱导的HAECs损伤的影响[J].中国实验方剂学杂志,2015(22):112-115.
• [7]Chen Y,Li D,Xu Y,et al.Essential oils from Fructus A.zerumbet protect human aortic endothelial cells from apoptosis induced by ox-LDL in vitro[J].Evid Based Complement Alternat Med,2014:956824-956833.
• [8]张彦燕,文波,陶玲,等.艳山姜挥发油对脂多糖诱导损伤的血管内皮细胞保护作用研究[J].中药药理与临床,2014(4):66-68.
• [9]徐旖旎,姜丰,李守巧.正交试验法优选大蝎子草总黄酮提取工艺[J].贵阳医学院学报,2016(1):41-44.
• [10]国家药典委员会.中华人民共和国药典[S].四部.北京:中国医药科技出版社,2015:203.
• [11]伍振峰,王赛君,杨明,等.中药挥发油提取工艺与装备现状及问题分析[J].中国实验方剂学杂志,2014(14):224-228.
• [12]Soichiro M,Koichi O,Takashi M.1,8-Cineole Ameliorates Steatosis of Pten Liver Specific KO Mice via Akt Inactivation[J].Int J Mol Sci,2015(16):12051-12063.
• [13]张彦燕,令狐克刚,陈妍,等.正交试验法研究艳山姜挥发油保护脂多糖诱导HUVEC损伤的活性组分[J].中国实验方剂学杂志,2014(20):9-12.

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