婴儿肝炎综合征肝脏巨细胞病毒低基质磷酸化蛋白的检测意义The Clinical Value of Hepatic Cytomegalovirus pp65 in Diagnosis of Infantile Hepatitis Syndrome
龙梅,朱莉,刘文莉,饶燕,庞勤辉,全小丽,张萧
LONG Mei,ZHU Li,LIU Wenli,RAO Yan,PANG Qinhui,QUAN Xiaoli,ZHANG Xiao
摘要(Abstract)：
目的:观察人巨细胞病毒(HCMV)低基质磷酸化蛋白(pp65)在婴儿肝炎综合征肝脏组织中的表达,为诊断婴儿巨细胞病毒性肝炎提供科学依据。方法:选择30例婴儿肝炎综合征患儿,在B超介导下进行肝组织活检,免疫组化法检测肝组织中HCMV pp65抗原,观察婴儿巨细胞病毒性肝炎特异性病理改变,同时用ELISA方法检测血清HCMV IgM水平,计算并比较两指标的阳性率。结果:30例婴儿肝炎综合征患儿肝组织HCMV pp65抗原阳性18例,血清IgM阳性6例,阳性率分别为60%、20%。两者阳性率比较差异有显著性(P<0.01)。结论:通过免疫组织化学检测婴儿肝组织中HCMV pp65抗原表达,可用于诊断婴儿巨细胞病毒性肝炎。
Objective: To observe the expression of low substrate phosphorylation protein( pp65) of human cytomegalovirus( HCMV) in liver tissues of infants with hepatitis syndrome,and so as to provide scientific basis for CMV-hepatitis diagnosis. Methods: Thirty infants with hepatitis syndrome were involved,liver biopsy was carried out under b-ultrasound guidance; HCMV pp65 antigen was detected with immunohistochemistry assay,HCMV-IgM level was detected with ELISA; The sensitivity of the two methods was compared. Results: Positive rates of HCMV pp65 antigen and HCMV-IgM were 60%( 18/30) and 20%( 6/30),respectively( P <0. 01). Conclusions: Testing liver HCMV pp65 antigen with immunohistochemistry assay can offer reliable evidence for CMV-hepatitis diagnosis,having clinical value.
关键词(KeyWords)：
巨细胞病毒;基质磷酸化蛋白;婴儿;肝炎综合征;肝;活组织检查
cytomegalovirus;pp65;infant;hepatitis syndrome;liver;biopsy
基金项目(Foundation): 贵阳市科技局计划项目(2012103)
作者(Author):
龙梅,朱莉,刘文莉,饶燕,庞勤辉,全小丽,张萧
LONG Mei,ZHU Li,LIU Wenli,RAO Yan,PANG Qinhui,QUAN Xiaoli,ZHANG Xiao
DOI: 10.19367/j.cnki.1000-2707.2014.01.021
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