赵海玉,邓云坤,方开云,杨淼
ZHAO Haiyu,DENG Yunkun,FANG Kaiyun,YANG Miao

• 1:贵州医科大学
• 2:贵州省人民医院麻醉科

摘要(Abstract)：

目的:观察褪黑素对带状疱疹后遗神经痛(PHN)大鼠CD4~+ CD25~+调节性T细胞表达的影响。方法:雄性SD大鼠60只,随机分为空白对照组(不做任何处理)、假手术组(仅暴露松果体)、PHN模型组(松果体切除后接种带状疱疹病毒)及治疗组(与PHN模型组一样造模,同时腹腔注射外源褪黑素);接种病毒30 d时进行机械痛觉评分,同时取大鼠外周血应用流式细胞术检测CD4~+ CD25~+调节性T细胞表达,应用RT-PCR技术检测Foxp3+基因表达,采用ELISA技术检测外周血TGF-β表达。结果:接种病毒30 d时,空白对照组与假手术组比较各项指标差异均无统计学意义(P>0.05);模型组和治疗组大鼠痛觉评分、外周血CD4~+ CD25~+调节性T细胞、Foxp3+基因和TGF-β表达均显著高于正常对照组(P<0.05);模型组各项指标均显著高于治疗组(P<0.05)。结论 :腹腔注射褪黑素可减轻带状疱疹病毒感染大鼠的PHN,其机制可能与下调CD4~+ CD25~+调节性T细胞表达改善机体免疫功能有关。
Objective: To observe the influence of melatonin on CD4~+ CD25~+ regulatory T cell expression in postherpetic neuralgia( PHN) rats. Method: Sixty male SD rats were randomly divided into 4 groups: blank control group( group B),sham-operation group( group S),PHN model group(group P),and treatment group(group T). Rats in group A were not dealt with specially. Conarium of rats in group B were exposed. Conarium of rats in group P were removed and the rats were inoculated with herpes zoster virus. Rats in group T were inject with exogenous menatonin intraperitoneally after received operations of group P. In 30 day after virus inoculation,mechanical pain was graded,expression of CD~4+ CD25~+ regulatory T cells in rat peripheral blood( PB) was tested with flow cytometry,Foxp3 + gene expression detected with RT-PCR technique,and PB TGF-β expression determined using ELLISA technology. Result: All of the indicators were not significantly different between groups B and S( P > 0. 05). The pain scores,expressions of PB CD~4+ CD25~+ regulatory T cells,Foxp3 +gene,and TGF-β were significantly higher in groups P and T than in group B( P < 0. 05),and were markedly higher in group P than in group T. Conclusion: Peritoneal cavity injection of melatonin could relieve PHN in herpes zoster virus infected rats. The mechanism might relate with the decrease of expression of CD4~+ CD25~+ regulatory T cells and the improvement of immunity.

关键词(KeyWords)： 褪黑素;带状疱疹;神经痛CD4~+ CD25~+调节性T细胞;大鼠,Sprague-Dawley
melatonin;herpes zoster;neuralgia;CD4~+ CD25~+ regulatory T cells;rats,Sprague-Dawley

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技计划(黔科合SY3122)

作者(Author): 赵海玉,邓云坤,方开云,杨淼
ZHAO Haiyu,DENG Yunkun,FANG Kaiyun,YANG Miao

DOI: 10.19367/j.cnki.1000-2707.2017.01.011

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