贵州医科大学学报

2018, v.43;No.212(05) 517-521

[打印本页] [关闭]
本期目录(Current Issue) | 过刊浏览(Past Issue) | 高级检索(Advanced Search)

骨康胶囊对SaOS-2人成骨样细胞增殖、分化及矿化的影响
Effects of Gukang Capsule on Cell Proliferation,Differentiation and Mineralization of Human Osteoblast-like SaOS-2 Cells

杨健;陆定艳;彭潇;董莉;沈艳珍;李勇军;席晓岚;刘亭;
YANG Jian;LU Dingyan;PENG Xiao;DONG Li;SHEN Yanzhen;LI Yongjun;XI Xiaolan;LIU Ting;Guizhou Provincial Key Laboratory of Pharmaceutics/State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University;School of Pharmacy,Guizhou Medical University;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM ( Ministry of Education) ,Guizhou Medical University;Guizhou Weikang Zifan Pharmaceutical Co.,Ltd;

摘要(Abstract):

目的:研究骨康胶囊对体外培养的SaOS-2成骨肉瘤细胞增殖、分化及矿化的影响。方法:培养成骨肉瘤细胞SaOS-2,设立对照组、不同浓度的骨康胶囊组(生药浓度为1、10、100、200、400、800 mg/L)及维生素D3组,采用MTT法检测SaOS-2细胞增殖能力;选择10、100及200 mg/L骨康胶囊浓度处理SaOS-2细胞,对-硝基苯磷酸盐法(PNPP法)检测SaOS-2细胞碱性磷酸酶(ALP)活性,ELISA法检测SaOS-2细胞骨钙素(OTC)和钙离子(Ca~(2+))分泌能力,采用细胞钙茜素红染色法对成骨细胞进行染色、观察SaOS-2细胞矿化能力。结果:与对照组相比,骨康胶囊在体外可以显著促进成骨肉瘤细胞的增殖(P<0.01),当骨康胶囊浓度为200 mg/L时作用最大,此后随着骨康胶囊浓度增大对SaOS-2细胞增殖的促进作用有明显减弱的趋势;在10、100、200 mg/L浓度范围内,骨康胶囊剂量依赖性地增加SaOS-2细胞ALP活性、促进OTC和Ca~(2+)的分泌及细胞矿化结节形成(P<0.05或P<0.01)。结论:骨康胶囊对体外培养的SaOS-2细胞的增殖、分化和矿化有明显的促进作用。
Objective: To study the effects of Gukang capsule on cell proliferation,differentiation and mineralization of human osteoblast-like SaOS-2 cells cultivated in vitro. Methods: SaOS-2 cells were cultivated. The control group,Gukang capsule group with different concentrations( The biological concentrations were 1,10,100,200,400 and 800 mg/L respectively) and Vitamin D3 group were set up,and cell proliferation was tested by MTT assay. The SaOS-2 cells were treated with the Gukang capsule( 10,100 and 200 mg/L respectively),and the Alkaline phosphatase( ALP) activity was measured by PNPP method. Secretion of osteocalcin( OTC) and Ca~(2+)was detected by ELISA assay.Osteoblasts were stained with calcium alizarin red staining method and mineralization of SaOS-2 cells was observed. Results: Compared with the control group,Gukang capsule could significantly promote proliferation of SaOS-2 cells in vitro( P < 0. 01),and increase ALP activity in a dose-dependent manner( P < 0. 01). Moreover,it could also increase the secretion of OTC and Ca~(2+)( P < 0. 05,P <0. 01),and significantly promote the formation of osteoblast mineralization nodules( P < 0. 05).Conclusions: Gukang capsule could increase cell proliferation,differentiation and mineralization activities in SaOS-2 cells cultivated in vitro,which may contribute to its therapeutic effects.

关键词(KeyWords): 骨质疏松症;骨康胶囊;成骨肉瘤细胞;增殖;分化;矿化;碱性磷酸酶;细胞骨钙素
osteoporosis;Gukang capsule;osteoscarlome cells;proliferation;differentiation;mineralization;alkaline phosphata;osteocalcin

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技计划项目[黔科合J重大字(2015)2002、黔科合LH字(2016)7373];; 贵州省教育厅项目[黔教合重大专项字(2015)040];; 贵州省创新人才团队项目[黔科合平台人才(2016)5613、黔科合平台人才(2016)5677]

作者(Author): 杨健;陆定艳;彭潇;董莉;沈艳珍;李勇军;席晓岚;刘亭;
YANG Jian;LU Dingyan;PENG Xiao;DONG Li;SHEN Yanzhen;LI Yongjun;XI Xiaolan;LIU Ting;Guizhou Provincial Key Laboratory of Pharmaceutics/State Key Laboratory of Functions and Applications of Medicinal Plants,Guizhou Medical University;School of Pharmacy,Guizhou Medical University;Engineering Research Center for the Development and Application of Ethnic Medicine and TCM ( Ministry of Education) ,Guizhou Medical University;Guizhou Weikang Zifan Pharmaceutical Co.,Ltd;

Email:

DOI: 10.19367/j.cnki.1000-2707.2018.05.005

参考文献(References):

文章评论(Comment):

序号(No.) 时间(Time) 反馈人(User) 邮箱(Email) 标题(Title) 内容(Content)
反馈人(User) 邮箱地址(Email)
反馈标题(Title)
反馈内容(Content)
扩展功能
本文信息
服务与反馈
本文关键词相关文章
本文作者相关文章
中国知网
分享