异氟烷对体外精子超微结构的影响Effects of Isoflurane on Ultrastructure of Human Sperm in Vitro
郑立宏,杨丹,高晓勤,丁贤胜,孙发
ZHENG Lihong,YANG Dan,GAO Xiaoqin,DING Xiansheng,SUN Fa
摘要(Abstract)：
目的:探讨异氟烷在体外对人类精子超微结构的影响。方法:收集24例精液常规正常的男性精子标本,每例精液均分成3份,分别给予1.1%异氟烷(A组)、2.2%异氟烷(B组)和空气(C组),并放置于孵箱培养5 h,采用扫描电镜观察各组精子的超微结构改变,计算异氟烷处理后顶体完整率、头部及尾部畸形率。结果:用异氟烷孵育后,头部及尾部畸形、顶体凹陷的精子数量增多;A、B组精子顶体完整率低于C组、头部及尾部畸形率高于C组,其中B组精子形态异常情况较A组更为严重,差异有统计学意义(P<0.05)。结论:异氟烷可影响精子形态,且随着异氟烷浓度增加,精子形态改变越来越明显。
Objective: To investigate the effect of isoflurane on ultrastructure of human sperm in vitro. Methods: 24 normal male sperm samples were collected. Each was divided into three groups and placed respectively in three tubes: 1. 1% isoflurane( group A),2. 2% isoflurane( group B) and air( group C). All of the tubes were placed in the incubator for 5 hours. The ultrastructural changes of sperms were observed by electron microscopy scanning to calculate the acrosome integrity rate,head and tail deformity rate. Results: After incubation with isoflurane,the number of sperms with head and tail deformity,and the acrosomatic depression increased. The sperm acrosome integrity rate in group A and B was lower than that in group C,and the head and tail deformity rate was higher than that in group C. The abnormal morphology of sperms in group B was more serious than that in group A. The difference was statistically significant( P < 0. 05). Conclusion: As the isoflurane concentration increases,changes of sperm morphology have become increasingly obvious with the integrity of sperm acrosome decreased and sperm deformity rate increased.
关键词(KeyWords)：
异氟烷;生殖功能;精子;超微结构;扫描电镜
isoflurane;reproductive function;sperm;ultrastructure;scanning electron microscope
基金项目(Foundation): 国家自然科学基金(81360097);; 贵州省科学技术厅研究项目[黔科合LH字(2014)7087]
作者(Author):
郑立宏,杨丹,高晓勤,丁贤胜,孙发
ZHENG Lihong,YANG Dan,GAO Xiaoqin,DING Xiansheng,SUN Fa
DOI: 10.19367/j.cnki.1000-2707.2018.07.003
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