徐峰,王莹
XU Feng,WANG Ying

• 1:南通市第一人民医院妇产科

摘要(Abstract)：

目的:探讨血清miR-210和miR-155水平对先兆子痫(PE)早期诊断的临床价值。方法:选取PE孕妇(PE组)和健康孕妇(对照组)各60例,收集所有孕妇的临床资料(年龄、孕周、身体质量指数、收缩压、舒张压、血红蛋白、尿素氮和肌酐等),采用实时定量荧光(PCR)法检测孕妇血清miR-210和miR-155的相对表达水平,采用接受者操作特性曲线(ROC)分析候选miRNA作为PE潜在诊断标志物的准确性。结果:两组孕妇的年龄、身体质量指数(BMI)、孕周、血红蛋白和尿素氮差异无统计学意义(P>0.05),PE组孕妇收缩压、舒张压和肌酐水平均明显高于对照组孕妇,差异有高度统计学意义(P<0.01);PE组孕妇血清miR-210和miR-155水平均高于对照组孕妇,差异有统计学意义(P<0.05);孕妇血清miR-210的ROC曲线下面积为0.904(95%CI为0.847～0.961,P<0.01),miR-155的ROC曲线下面积为0.843(95%CI为0.766～0.920,P<0.01),孕妇血清miR-210水平用于预测PE的灵敏度和特异性均高于miR-155。结论:孕妇血清miR-210和miR-155水平可为PE的早期诊断提供参考,miR-210的诊断价值高于miR-155。
Objective: To investigate the clinical value of serum miR-210 and miR-155 levels as early diagnostic markers of pre-eclampsia(PE). Methods: Sixty pregnant women with PE(PE group) and sixty healthy pregnant women(control group) were enrolled. The clinical data of all pregnant women(age, gestational age, body mass index(BMI), systolic blood pressure, diastolic blood pressure, hemoglobin, urea nitrogen and creatinine) were collected. Real-time quantitative PCR(qPCR) was used to detect the relative expression levels of maternal serum miR-210 and miR-155, and the receiver operating characteristic curve(ROC) was used to analyze the accuracy of candidate miRNAs as potential diagnostic markers for PE. Results: There was no significant difference in age, BMI, gestational age, hemoglobin and urea nitrogen between the two groups(P>0.05). The systolic blood pressure, diastolic blood pressure and creatinine level in the PE group were significantly higher than those in the control group(P<0.01). The serum levels of miR-210 and miR-155 in the PE group were higher than those in the control group(P<0.05). The area under the ROC curve of serum miR-210 in pregnant women was 0.904(95% CI 0.847～0.961, P<0.01), and that of miR-155 was 0.843(95% CI 0.766～0.920, P<0.01). The sensitivity and specificity of The serum miR-210 level to predict PE are higher than that of miR-155 in pregnant women. Conclusion: The serum levels of miR-210 and miR-155 in pregnant women can be biomarkers for early diagnosis of PE. The diagnostic value of miR-210 is higher than that of miR-155.

关键词(KeyWords)： 先兆子痫;早期诊断;微RNAs;ROC曲线;标志物
pre-eclampsia;early diagnosis;microRNA;ROC curve;biomarkers

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 徐峰,王莹
XU Feng,WANG Ying

DOI: 10.19367/j.cnki.1000-2707.2019.09.021

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