贵州医科大学学报

2021, v.46;No.254(11) 1289-1293+1300

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白蛋白紫杉醇与五氟尿嘧啶联合用药对人胃癌细胞增殖及周期的影响
Effects of albumin-bound paclitaxel combined with 5-fluorouracil on proliferation and cycle of gastric cancer cells

成兴真;杨芳;王杨;聂微;刘泽莹;王黔;严芝强;
CHENG Xingzhen;YANG Fang;WANG Yang;NIE Wei;LIU Zeying;WANG Qian;YAN Zhiqiang;Department of Surgery,Guizhou Medical University;Department of Clinical Hematology,School of Medical Laboratory Science,Guizhou Medical University;Center of Clinical Laboratory,the Affiliated Hospital of Guizhou Medical University;Department of Gastrointestinal Surgery,the Affiliated Hospital of Guizhou Medical University;

摘要(Abstract):

目的探索白蛋白紫杉醇(Nab-PTX)与五氟尿嘧啶(5-Fu)联合用药对人胃癌细胞(AGS)增殖及周期的影响。方法对数生长的AGS细胞分为对照组(RMPI 1640, Ctrl组)、Nab-PTX组(Nab-PTX,浓度梯度为0.1、0.2、0.4μmol/L)、5-Fu组(5-Fu,浓度梯度为1、2、4μmol/L)及Nab-PTX+5-Fu组[Nab-PTX+5-Fu,浓度梯度为(0.1+1)、(0.2+2)、(0.4+4)μmol/l];相应药物作用24、48以及72 h,采用CCK-8法检测各组细胞增殖能力,流式细胞术检测细胞周期。结果与Ctrl组相比,Nab-PTX组、5-Fu组、Nab-PTX+5-Fu组细胞增殖能力均受到抑制(P<0.05),且联合用药抑制率较单药明显增加,同时Nab-PTX、5-Fu、Nab-PTX+5-Fu作用于AGS细胞株时抑制率随时间延长而递增;与Ctrl组相比,Nab-PTX组细胞周期阻滞于G2/M期(P<0.05),5-Fu组细胞周期阻滞于S期(P<0.05),Nab-PTX+5-Fu组细胞周期阻滞在S期(P<0.01)。结论 Nab-PTX与5-Fu联合用药较两药单独应用能更有效地抑制细胞增殖,同时也使细胞周期阻滞在S期。
Objective To explore the effect of combined administration of albumin-bound paclitaxel(Nab-PTX) and 5-fluorouracil(5-Fu) on proliferation and cycle of human gastric cancer cells(AGS).Methods AGS at logarithmic growth were divided into control group(RMPI 1640,Ctrl group),Nab-PTX group(Nab-PTX at the concentration gradients of 0.1,0.2,0.4 μmol/L),5-Fu group(5-Fu at the concentration gradients of 1,2,4 μmol/L) and the Nab-PTX+5-Fu group [NabPTX+5-Fu,the concentration gradient is(0.1+1),(0.2+2),(0.4+4) μmol/1].After 24,48,and 72 hours of treatment,CCK-8 assay was used to detect cell proliferation in each group,and flow cytometry was used to detect cell cycle.Results Compared with the Ctrl group,the cell proliferation was inhibited in Nab-PTX,5-Fu group and Nab-PTX+5-Fu groups(P <0.05).The inhibition rate was significantly increased in Nab-PTX+5-Fu group when compared with individual drugs.At the same time,the inhibition rates in Nab-PTX,5-Fu,and Nab-PTX+5-Fu were time-dependent.Compared with the Ctrl group,Nab-PTX group was arrested at G2/M phase(P <0.05),5-Fu group at in S phase(P <0.05) and Nab-PTX+5-Fu group at S phase(P <0.01).Conclusion The combination of Nab-PTX and 5-Fu can inhibit cell proliferation more effectively than the two drugs alone and block the cell cycle in S phase.

关键词(KeyWords): 胃癌细胞;白蛋白紫杉醇;五氟尿嘧啶;细胞增殖;细胞周期;联合用药
gastric cancer cells;albumin-bound paclitaxel(Nab-PTX);5-fluorouracil(5-Fu);cell proliferation;cell cycle;drug combination

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技计划项目学术新苗(19NSP015)

作者(Authors): 成兴真;杨芳;王杨;聂微;刘泽莹;王黔;严芝强;
CHENG Xingzhen;YANG Fang;WANG Yang;NIE Wei;LIU Zeying;WANG Qian;YAN Zhiqiang;Department of Surgery,Guizhou Medical University;Department of Clinical Hematology,School of Medical Laboratory Science,Guizhou Medical University;Center of Clinical Laboratory,the Affiliated Hospital of Guizhou Medical University;Department of Gastrointestinal Surgery,the Affiliated Hospital of Guizhou Medical University;

DOI: 10.19367/j.cnki.2096-8388.2021.11.008

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