方艺,于燕妮,胡军
FANG Yi,YU Yanni,HU Jun(Department of Pathology

• 1:贵阳医学院病理学教研室
• 2:贵阳医学院病理学教研室
• 3:贵阳医学院病理学教研室 贵州贵阳550004
• 4:贵州贵阳550004

摘要(Abstract)：

目的:探讨p53与Ki67在胃癌中的表达及临床意义。方法:应用免疫组织化学Envision二步法检测25例胃癌组织中p53与Ki67的表达水平。结果:25例胃癌中乳头状腺癌-管状腺癌(分化较好)14例,低分化腺癌-印戒细胞癌(分化较差)11例。p53与Ki67随胃癌的分化程度降低和分期的增加,阳性表达率递增(P<0.05)。结论:胃癌组织中p53高表达与胃癌组织细胞增殖密切相关,且p53与Ki67在胃癌中存在相关差异,通过检测p53与Ki67的表达,有助于胃癌预后的评估。
Objective:To investigate the expression of p53 and ki67 in gastric carcinom and its significance.Methods: The Envision immunohistochemical method was applied to check the expression of p53 and ki67 in 25 case of gastric carcinoma(14 cases highly differentiated,11 cases poorly differentiated).Results: The positive expression rates of p53 and ki67 were both much higher in poorly differentiated cases than in highly differentiated cases.The lower the differential degree and the higher the stage,the higher the positive expression rate(P<0.05).Conclusions: High expression of p53 might have some relationship to proliferative activity of gastric carcinoma.The results suggest that immunological labeling of p53 and ki67 might be useful for prognosis determination of gastric carcinoma.

关键词(KeyWords)： 胃肿瘤;基因,p53;免疫组织化学
stomach neoplasms;genes,p53;immunohistochemistry

Abstract:

Keywords:

基金项目(Foundation): 贵州省优秀科技人才省长基金资助项目(s2004-10)

作者(Author): 方艺,于燕妮,胡军
FANG Yi,YU Yanni,HU Jun(Department of Pathology

DOI: 10.19367/j.cnki.1000-2707.2007.01.009

参考文献(References)：

• [1]Mazar GH,Jeanteur P.Nucleotide sequence polymorphismin a hotspot mutation region of the p53 gene[J].Onco-gene,1992(4):781-782.
• [2]Milner J,Medcalf EA.Cotranslation of activated mutantp53 with wild type drives the wild-type p53 protein intothe mutant conformation[J].Cell,1991(65):765-774.
• [3]Finlay Ca,Hinds PW,Tan TH.Activating mutations fortransformation by p53 produce a gene product that formsan hsc70-p53 complex with an altered half life[J].J Vir-ol,(8):531-539.
• [4]耿明,曹永成.胃癌胃粘膜异型增生和肠上皮化生P16和P53蛋白的表达[J].诊断病理学杂志,2003(1):34-35.
• [5]李其云,牛凤霞.胃癌细胞凋亡与增殖的观察[J].临床与实验病理学杂志,2001(3):205-208.
• [6]王文亮,张传山.胃粘膜活检中粘液细胞癌的临床病理特点[J].诊断病理学杂志,2002(1):34.
• [7]李宁.增值细胞核抗原与胃癌预后的关系[J].中华病理杂志,1993(7):545.
• [8]葛少华,刘宝瑞,钱晓萍.顺铂与热疗联合对胃癌细胞系体外增殖抑制作用的研究[J].实用临床医药杂志,2006(3):4-7.
• [9]sawhney N,Hall PA.Ki67-structure function and newanti-bodies[J].J Pathol,1992(168):161-162.
• [10]Sasaki k,Murakami T.The cell associated change of theKi67reactive nuclear antigen expression[J].J cell Physi-ol,1987(3):579-584.

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