王继芬,康朝胜,臧贵勇,李强明
WANG Jifen1,KANG Chaosheng2,ZANG Guiyong2,LI Qiangming2(1.Department of Neurology

• 1:黔西南州人民医院神经内科
• 2:贵阳医学院人体解剖学教研室

摘要(Abstract)：

目的:从慢性铝中毒大鼠海马CA3区胆碱乙酰转移酶(Choline Acetytransferase,ChAT)变化的角度,探讨铝的神经毒性作用机制。方法:40只4～5月龄SD大鼠,随机分为铝中毒组和正常对照组,采用尼氏染色及ChAT免疫组化染色,计数阳性细胞,用细胞形态学计量方法测量ChAT阳性产物的平均光密度。结果:铝中毒组大鼠海马CA3区ChAT阳性神经元减少,合成的乙酰胆碱(Acetychdine,Ach)也减少。结论:铝可对大鼠海马CA3区ChAT阳性神经元产生神经毒性作用。
Objective: To probe the mechanism of aluminum neurotoxic effect by observing the changes of choline acetytransferase(ChAT) in hippocampal CA3 of chronically aluminum poisoned rats.Methods: Forty SD rats aged 4～5 months,were randomly divided into 2 groups: control group(group C) and aluminium toxicity group(group A).Rats in group A were given 1.0% aluminum chloride intragastrically(100 mg/kg)daily for 3 months,and rats in group C were given distilled water instead.By the end of the experiment,sections of rat hippocampus were stained with Nissl's dyeing and immunohistochemical staining,positive cells were counted,and average optical of ChAT product was measured with morphological measuring methods.Results: Number of ChAT positive neurons in hippocampal neuronal CA3 area in group A was smaller than that in group C,and ChAT product,acetychdine(Ach),was less in group A than that in group C.Conclusion: Aluminum is toxic to ChAT positive neurons in hippocampal CA3 area of rats.

关键词(KeyWords)： 铝中毒;海马CA3区;神经元;胆碱乙酰转移酶;免疫组织化学;大鼠
aluminum poisoning;hippocampal CA3 area;neurons;choline acetyltransferase;immunohistochemistry;rats

Abstract:

Keywords:

基金项目(Foundation): 贵州省教育厅科技基金项目(F2006)

作者(Author): 王继芬,康朝胜,臧贵勇,李强明
WANG Jifen1,KANG Chaosheng2,ZANG Guiyong2,LI Qiangming2(1.Department of Neurology

DOI: 10.19367/j.cnki.1000-2707.2010.06.005

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