赵亮,陈峥宏,王菲
ZHAO Liang,CHEN Zhenghong,WANG Fei (Department of Microbiology

• 1:贵阳医学院微生物学教研室

摘要(Abstract)：

目的:提取及纯化SD大鼠肺灌洗液中表面活性物质结合蛋白D(SP-D)。方法:取SD大鼠肺泡灌洗液,经Tris-HCl-EDTA法提取和maltose-agarose亲和层析法纯化SP-D,用SDS-PAGE电泳及SP-D与幽门螺杆菌的玻片凝集试验鉴定。结果:从SD大鼠肺灌洗液中提取出较高浓度的蛋白,经SDS-PAGE电泳检测,分子量约43 kD,提取的蛋白可与幽门螺杆菌发生明显凝集。结论:采用maltose-agarose亲和层析法可以从SD大鼠肺灌洗液中获得较高纯度的SP-D。
Objective: To isolate and purify surfactant protein D(SP-D) from SD rat bronchoalveolar lavage liquid.Metheds: SP-D were eluted from bronchoaveolar lavage fluid of SD mice with Tris-HCl-EDTA,purified by maltose-agarose affinity chromatography,and then determined by SDS polyacrylamide gel electrophresis(SDS-PAGE) and agglutination test with Helicobacter pylori.Results: SP-D with high concentration was obtained by maltose-agarose affinity chromatography.The molecular weight of the obtained SP-D was 43kDa.Helicobacter pylori could be agglutinated by the protein.Conclusion: Highly pure SP-D could be obtained from SD rat bronchoalveolar lavage liquid by affinity chromatography on maltose-agarose.

关键词(KeyWords)： 肺泡表面活性物质相关蛋白质D;大鼠,Sprague-Dawley;组织提取物;方法;生物学鉴定法
Pulmonary surfactant-associated proteins D;rats,Sprague-Dawley;tissue extracts;methods;biological assay

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅基金资助项目[E2008-4]

作者(Author): 赵亮,陈峥宏,王菲
ZHAO Liang,CHEN Zhenghong,WANG Fei (Department of Microbiology

DOI: 10.19367/j.cnki.1000-2707.2009.03.012

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