贵州医科大学学报

2016, v.41;No.193(10) 1193-1196

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家蝇幼虫干粉对小鼠血液部分生理生化指标和免疫功能的影响
Effect of Housefly Larva Powder on Blood Physiological and Biochemical Indexes and Immune Function of Mice

魏洪;夏冰天;迟茜文;文雪;
WEI Hong;XIA Bingtian;CHI Qianwen;WEN Xue;Department of Microbiology,Laboratory for Infection and Immunology Experiments,Guizhou Medical University;School of Clinical Medicine,Guizhou Medical University;

摘要(Abstract):

目的:探讨家蝇幼虫干粉对小鼠血液部分生理生化指标及免疫功能的影响。方法:在小鼠基础饲料中添加5%、7.5%、9%家蝇幼虫干粉喂养30 d,同时设立基础饲料喂养的对照组,取血检测小鼠血红蛋白(Hb)、红细胞(RBC)、白细胞(WBC)和血小板(PLT)、天门冬氨酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、尿素氮(BUN)和肌酐(Cr),通过免疫器官指数、碳粒廓清试验、脾细胞介导的SRBC分光光度计定量测定法(QHS),外周血T淋巴细胞ANAE+阳性率测定评价小鼠免疫功能。结果:与对照组相比,5%家蝇幼虫干粉组ALT含量提高(P<0.05),AST含量降低(P<0.05);7.5%家蝇幼虫干粉组脾指数高于对照组,5%、9%家蝇幼虫干粉组脾指数低于对照组(P<0.05),5%、7.5%和9%家蝇幼虫干粉组胸腺指数均高于对照组(P<0.05);9%家蝇幼虫干粉组吞噬指数α、QHS反应值和ANAE+阳性率均显著高于对照组(P<0.01)。结论:高剂量家蝇幼虫干粉具有增强免疫作用,低剂量可能影响血清生化指标。
Objective: To study the influence of housefly larva( Musca domestica L) powder on blood physiological and biochemical indexes and immune function of mice. Methods: Housefly larva powder with the concentration of 5%,7. 5% and 9% was added in basal feed to feed mice for 30 days while the control group received only basal feed. The blood of mice was collected to detect Hb,RBC,WBC,PLT,ALT,AST,creatinine( Cr) and blood urea nitrogen( BUN). The immune organ index,carbon clearance test,spleen cell mediated SRBC spectrophotometry measure( QHS),the positive rate of ANAE+in peripheral blood lymphocytes were determined to evaluate the immune function of mice. Results: Compared with the control group,the content of ALT increased in the group of 5%concentration of housefly larvae powder( P < 0. 05) while the content of AST decreased( P < 0. 05);In the group of 7. 5% concentration of housefly larvae powder spleen index was higher than that of the control group,while in the group of 5% and 9% concentration of housefly larvae powder spleen index was lower than that of the control group( P < 0. 05). In the group of 5%,7. 5% and 9% concentration of housefly larvae powder,thymus index was higher than that of the control group( P < 0. 05). In the group of 9% concentration of housefly larvae powder group,phagocytic index alpha,QHS response value and ANAE + positive rate were significantly higher than their counterparts of control group( P <0. 01). Conclusion: High dose of housefly larva powder can promote immunity function of mice,while low dose of powder might affect blood biochemical indexes.

关键词(KeyWords): 家蝇;幼虫;小鼠;血液学;肝功能试验;肾功能试验;免疫功能
housefly;larva;mice;haematology;liver function test;renal function test;immunity function

Abstract:

Keywords:

基金项目(Foundation): 地方高校国家级大学生创新创业训练计划项目(201510660010);; 贵州省大学生创新创业训练计划项目(201410660019)

作者(Author): 魏洪;夏冰天;迟茜文;文雪;
WEI Hong;XIA Bingtian;CHI Qianwen;WEN Xue;Department of Microbiology,Laboratory for Infection and Immunology Experiments,Guizhou Medical University;School of Clinical Medicine,Guizhou Medical University;

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DOI: 10.19367/j.cnki.1000-2707.2016.10.017

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