简单,温丽娜,孙朝琴,莫非,李永念
JIAN Dan,WEN Lina,SUN Chaoqin,MO Fei,LI Yongnian

• 1:贵州医科大学
• 2:贵阳中医学院第二附属医院

摘要(Abstract)：

目的:检测头花蓼对幽门螺杆菌(H.pylori)感染SD大鼠胃粘膜胃泌素(Gas)和生长抑素(SS)表达的影响,探讨头花蓼抗H.pylori感染性胃炎的作用机制。方法:42只SD大鼠随机分为造模组26只和空白组16只,采用H.pylori标准菌灌胃SD大鼠建立H.pylori慢性胃炎大鼠模型;造模成功后,造模大鼠分为模型组和头花蓼组,头花蓼组以1.72 g浸膏/(kg·d)的剂量灌胃给药,模型组和空白组大鼠灌胃等量生理盐水,治疗2周后处死大鼠,取胃窦黏膜组织,HE染色观察大鼠胃黏膜组织学改变;免疫组织化学染色观察胃黏膜Gas阳性细胞和SS阳性细胞的改变,原位杂交检测大鼠胃黏膜Gas和SS mRNA的表达水平。结果:与模型组比较,头花蓼可明显改善H.pylori感染大鼠胃黏膜炎症,病理评分显著降低(P<0.05);头花蓼组大鼠胃黏膜Gas阳性细胞数量明显减少,Gas mRNA表达水平明显下降,差异具有统计学意义(P<0.05);SS阳性细胞数量、SS mRNA表达水平较模型组比较,差异无统计学意义(P>0.05)。结论:头花蓼可修复H.pylori感染慢性胃炎胃黏膜损伤,其机制可能与改善胃肠激素对胃功能调节有关。
Objective:To investigate the effect of Polygonum capitatum on gastric mucosal gastrin(Gas) and somatostatin(SS) expression of SD rats infected by H.pylori and to explore the mechanism of Polygonum capitatum against H.pylori infection gastritis.Methods:42 rats were divided into model group of 26 rats and blank control group of 16 rats.Establishment of H.pylori rat model of chronic gastritis was established in rats by intragastric administration of H.pylori standard strain.After successful modeling,rats were divided into model group and Polygonum capitatum group.Polygonum capitatum group was given 1.72 g extractum/kg dose of Polygonum capitatum by intragastric administration while model group and blank group were given equal volume of saline by intragastric administration.After 2weeks of treatment,the rats were killed,the gastric mucosa was taken and the histological changes of gastric mucosa were observed by HE staining.Immunohistochemical staining was used to observe the changes of Gas positive cells and SS positive cells in gastric mucosa.The expression levels of mRNA Gas and mRNA SS in gastric mucosa were detected by in situ hybridization.Results:Compared with the model group,Polygonum capitatum can significantly alleviate the inflammation of the gastric mucosa of rats infected with H.pylori,and the pathological score decreased significantly(P<0.05).The number of Gas positive cells of gastric mucosa in Polygonum capitatum group significantly decreased and the expression level of Gas mRNA decreased significantly.The difference was statistically significant(P<0.05).There was no statistically significant difference in the number of SS positive cells and mRNA SS expression level between the model group and Polygonum capitatum group(P>0.05).Conclusion:Polygonum capitatum can repair gastric mucosal damage in chronic gastritis infected with H.pylori,and its mechanism may be related to improving the stomach intestine hormone regulation.

关键词(KeyWords)： 螺杆菌,幽门;胃炎;头花蓼;胃泌素;生长抑素
Helicobacter pylori;gastritis;Polygonum capitatum;gastrin;somatostatin

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金[黔科合(2014)2027号];; 贵州省科技合作计划基金[黔科合LH(2015)7417号];; 贵州省卫生计生委科学技术基金(gzwikj2015-1-003);; 贵阳市科技局计划项目[筑科合同(2014001)]

作者(Author): 简单,温丽娜,孙朝琴,莫非,李永念
JIAN Dan,WEN Lina,SUN Chaoqin,MO Fei,LI Yongnian

DOI: 10.19367/j.cnki.1000-2707.2016.12.008

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