李玲,陈如筑
LI Ling~1; CHEN Ru-zhu~2(1.Department of Pharmacology;

• 1:贵阳医学院药理学教研室
• 2:中山医科大学药理学教研室 贵州贵阳550004
• 3:广东广州510089

摘要(Abstract)：

目的:观察二氢麦角毒对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)帕金森病模型小鼠行为学、脑超氧化物歧化酶(SOD)活性及纹状体多巴胺及其代谢产物含量的影响。方法:小鼠腹腔注射MPTP 30 mg/kg,2次/d,连续5 d致帕金森模型,用YSD-4G药理生理实验多用仪测定小鼠自发活动记数,用高效液相色谱(HPLC)法测定纹状体内多巴胺及其代谢产物含量,酶法测脑SOD活性。结果:二氢麦角毒能明显增加MPTP帕金森病模型小鼠的自发活动、脑SOD活性和纹状体内多巴胺及其代谢产物含量。结论:二氢麦角毒能改善MPTP帕金森病模型小鼠的自发活动减少的症状,其机制可能与增加脑SOD活性及纹状体内多巴胺含量有关。
Objective: To observe the effects of dihydroergotoxine on parkinsonism model mice including their behavior, activity of brain SOD, and the contents of dopamine and derivatives in striate body. Methods: Parkinsonism model mice were established by giving mice with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (ip) at a dosage of 30mg/kg, twice a day for 5 days. The locomotor activity was measured on the 7th and 14th days afterwards. The contents of dopamine, dihydroxyphenylacetic acid and homovanillic acid in striatum were measured with LPHC, and SOD activity in brain tissue was measured with enzyme method. Results: The locomotor activity, and the contents of dopamine, dihydroxyphenylacetic acid, homovanillic acid and SOD increased significantly in dihydroergotoxine groups. Conclusion: Dihydroergotoxine can remarkably enhance the locomotor activity of the MPTP-treated mice, which might relate with increase of dopamine, dihydroxyphenylacetic acid, homovanillic acid and SOD in brain tissue.

关键词(KeyWords)： 帕金森病;多巴胺;二氢麦角毒;模型,动物;小鼠
Parkinson disease; dopamine; dihydroergotoxine; models,animal; mice

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 李玲,陈如筑
LI Ling~1; CHEN Ru-zhu~2(1.Department of Pharmacology;

DOI: 10.19367/j.cnki.1000-2707.2005.04.011

参考文献(References)：

• [1]Boism are F,M icheli I.E ffects of d ihydroergotoxine andeach of its componenets on cerebral ischem ia in the cats[J].J Hparm acol,1974,5(2):221-230.
• [2]Cuc inotta D,De Leo D,Frattola L.D ihydroergocryptine vsp lacebo indem intia of A lzhe im er type:interim resu lts of arandom ized mu lticenter study after a 1 year follow up[J].Arch ives of gerontology and geriatrics,1996,22(1):169-180.
• [3]Bonuccelli U,D’Anton io P,D’Avino C.D ihydroergocryp-tine in the treatm ent of Park inson d isease[J].J Neural tre-ansm,1995,45:239-245.
• [4]Nobutaka Arai,Kazuak i M isugi,Yosh io Gosh im a,et al.E-valuation of a 1-4-phenyl-1,2,3,6-tetrahydropyrid ine(MPTP)-treated C57 b lack mouse model for park inson ism[J].B rain Research,1990,515:57-63.
• [5]徐修容,唐琴梅,姚一禾.以D-樟脑-β磺酸为离子对试剂反相高效液相色谱测定大鼠脑组织中生物胺及其代谢产物[J].中国药理学报,1987,8(2):113-117.
• [6]B lum e E.Street drugs yield prim ate Park inson’s model[J].JAMA,1983,250:13.
• [7]Russ H,StaustK,M artel F,et al.The extraneuronal trans-porter for monoam ine transm itters exists in cells derivedfrom hum an central nervous system glia[J].Eur J Neuros-c i,1996,8:1 256-1 264.

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