赵哲仪,王正蓉,方兴艳,王甜,罗昭逊,谢婷婷
ZHAO Zheyi,WANG Zhengrong,FANG Xingyan,WANG Tian,LUO Zhaoxun,XIE Tingting

• 1:贵州医科大学附属医院临床检验中心
• 2:贵州医科大学医学检验学院
• 3:贵州医科大学
• 4:贵州医科大学附属医院产前诊断中心

摘要(Abstract)：

目的探讨亚砷酸钠(NaAsO_2)对小鼠肝细胞AML12损伤作用的机制。方法取对数生长期的小鼠正常肝细胞AML12,设置6个NaAsO_2浓度[0(对照)、10、15、20、25及30μmol/L]组,分别用相应浓度的NaAsO_2处理AML12细胞24 h;采用化学比色法和油红O染色法检测各组AML12细胞内丙二醛(MDA)和脂质含量水平,采用实时荧光定量PCR(RT-qPCR)和Western blot法分别检测各组小鼠AML12细胞内法尼醇X受体(FXR)、小异二聚体伴侣(SHP)mRNA和FXR、SHP、Bcl-2相关X蛋白(Bax)蛋白的表达。结果与对照组相比,10～30μmol/L NaAsO_2组小鼠AML细胞内MDA含量升高、但FXR和SHP mRNA及FXR蛋白相对表达量下降(P<0.05),15～30μmol/L NaAsO_2组小鼠AML细胞内脂滴含量增加(P<0.05),20～30μmol/L NaAsO_2组小鼠AML细胞内SHP蛋白相对表达量降低(P<0.05),25～30μmol/L NaAsO_2组小鼠AML细胞内BAX蛋白相对表达量增高(P<0.05)。结论 NaAsO_2可致小鼠肝细胞AML12损伤,其机制可能与FXR-SHP信号通路的失调有关。
Objective To investigate the mechanism of injury induced by sodium arsenite(NaAsO_2 on mouse hepatocytes AML12.Methods Mouse normal hepatocytes AML12 at logarithmic growth stage were taken and treated with [0(control), 10, 15, 20, 25,and 30 μmol/L]NaAsO_2 for 24 h.The contents of intracellular Malondialdehyde(MDA) and lipid droplets in AML12 cells were detected by chemical colorimetry and oil red O staining. The expression of steatosis; farnesoid X receptor(FXR), small heterodimer partner(SHP) mRNA and FXR, SHP, and Bcl-2 related X protein(Bax)were detected by real-time fluorescence quantative PCR and western blot.Results Compared with the control group, MDA content in 10-30 μmol/L NaAsO_2 groups increased(P< 0. 05), but the relative expression levels ofFXR,SHPmRNA and the relative expression levels of FXR protein decreased(P<0. 05). Compared with the control group, the lipid droplets content in 15-30 μmol/L NaAsO_2 groups increased(P< 0. 05), the relative expression of SHP protein in 20-30 μmol/L NaAsO_2 groups decreased(P< 0. 05), the relative expression of BAX protein in 25-30 μmol/L NaAsO_2 groups increased(P< 0. 05).Conclusion NaAsO_2 induces mouse hepatocytes AML12 injury which may be related to the dysregulation of FXR-SHP signaling pathway.

关键词(KeyWords)： 细胞凋亡;亚砷酸钠;小鼠肝细胞AML12;氧化应激;脂肪变性;法尼醇X受体;小异二聚体伴侣
apoptosis;sodium arsenite(NaAsO_2);mouse hepatocyte AML12;oxidative stress;steatosis;farnesoid X receptor(FXR);small heterodimer partner(SHP)

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（81560514）;; 贵州省科技创新人才团队项目（2019-5610）

作者(Author): 赵哲仪,王正蓉,方兴艳,王甜,罗昭逊,谢婷婷
ZHAO Zheyi,WANG Zhengrong,FANG Xingyan,WANG Tian,LUO Zhaoxun,XIE Tingting

DOI: 10.19367/j.cnki.2096-8388.2022.01.001

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