叶惠平,易烛光,龚正鹏
YE Huiping,YI zhuguang,GONG zhengpeng

• 1:贵州医科大学附属白云医院耳鼻咽喉科
• 2:贵州医科大学附院耳鼻咽喉科
• 3:贵州省人民医院耳鼻咽喉科

摘要(Abstract)：

目的:初步探讨长链非编码RNA(lnc RNA)在研究头颈鳞癌(HNSCC)放疗中的作用。方法:将HNSCC-SCCⅦ细胞分为对照组(不予放疗)和实验组(予放疗干预),应用芯片技术,筛选出放疗干预后HNSCCSCCⅦ细胞中差异表达的lnc RNA及m RNA,通过Cis和Trans法对靶基因进行预测,应用DAVID软件进行KEGG通路筛选,得到对应的靶基因富集的KEGG通路包括肿瘤相关通路、Wnt信号通路及轴突导向通路,分析这3条通路上对应的靶基因,并找到与这些靶基因对应的lnc RNA。结果:筛选出1 280条放疗差异表达的lnc RNA,对照组相对实验组高表达5倍以上的共15条,低表达5倍以上的共8条;筛选发现表达差异的m RNA有1 087条,对照组相对实验组高表达的528条,对照组相对实验组低表达559条;KEGG通路信息分析lnc RNA对应的靶基因富集于肿瘤相关通路、Wnt信号通路和轴突导向通路;Wnt信号通路中靶基因PRKCA和STAT5B对应的lnc RNA与m RNA是共表达的,对应的16203-3lnc RNA在对照组的表达为实验组的3.176倍(P<0.05),对应的31238-3lnc RNA在实验组中的表达量为对照组中的2.037倍(P<0.05)。结论:PRKCA和STAT5B可能是控制HNSCC放疗反应敏感性的靶基因,对应的16203-3lnc RNA和31238-3lnc RNA可作为调控HNSCC放疗敏感性的候选lnc RNA。
Objective: To preliminarily explore the function of long non-coding RNA(lnc RNA) in radiotherapy of head and neck squamous cell carcinoma( HNSCC). Methods: HNSCC-SCC Ⅶ cell strains were divided into control group( no radiotherapy) and experimental group( radiotherapy intervention). Chip technology was adopted to screen out the lnc RNA and m RNA of differential expression from HNSCC-SCCⅦ cells after radiotherapy intervention. CIS and Trans methods were used to predict target gene. DAVID analyzing software were used for KEGG pathway screening. Then,the corresponding KEGG pathway with target gene enrichment was obtained,including tumor related pathway,Wnt signal pathway and axon guidance pathway. The corresponding target genes in the three pathway were analyzed and the lnc RNAs corresponding to the target genes were found. Results: 1 280 differentially expressed lnc RNAs were screened out from HNSCC-SCCⅦ cell strains after radiotherapy intervention.Compared with experimental group,in control group there were totally 15 lnc RNAs above 5 times high expression while there were 8 lnc RNAs above 5 times low expression. There were 1 087 m RNAs of differential expression,of which there were 528 m RNAs relatively high expression in control group compared with experimental group while there were 559 m RNAs relatively low expression in control group compared with experimental group. KEGG signal pathway analysis showed that the target genes corresponding to lnc RNAs were enriched in tumor related pathway,Wnt signal pathway and axon guidance pathway. The lnc RNAs and m RNAs corresponding to target gene PRKCA and STAT5 B in Wnt signal pathway were co-expressed. The expression level of 16203- 3 lnc RNA in control group was 3. 176 times as much as in experimental group while the expression level of 31238- 3 lnc RNA in experimental group was 2. 037 times as much as in control group. Conclusion: PRKCA and STAT5 B might be the sensitive target gene controling HNSCC radiotherapy reaction. The two lnc RNA corresponding to them,16203- 3lnc RNA and 31238- 3 lnc RNA can be taken as candidate lnc RNA regulating the radiosensitivity.

关键词(KeyWords)： RNA,肿瘤;芯片;头颈部肿瘤;KEGG通路;放疗
RNA;carcinoma;chip;head and neck carcinoma;KEGG pathway;radiotherapy

Abstract:

Keywords:

基金项目(Foundation): 贵州省科学技术基金[黔科合LG(2012-068)号]

作者(Author): 叶惠平,易烛光,龚正鹏
YE Huiping,YI zhuguang,GONG zhengpeng

DOI: 10.19367/j.cnki.1000-2707.2016.02.023

参考文献(References)：

• [1]Cripps C,Winquist E,Devries MC,et al.Epidermal growth factor receptor targeted therapy in stagesⅢandⅣhead and neck cancer[J].Current Oncology,2010(3):37-48.
• [2]Jemal A,Siegel R,Ward E,et al.Cancer statistics,2008[J].CA Cancer J Clin,2008(58):71-96.
• [3]Cripps C,Winquist E,Devries MC,et al.Epidermal growth factor receptor targeted therapy in stagesⅢandⅣhead and neck cancer[J].Current Oncology,2010(3):37-48.
• [4]Delaney G,Jacob S,Featherstone C,et al.The role of radiotherapy in cancer treatment:estimating optimal utilization from a review of evidence based clinical guidelines[J].Cancer,2005(104):1129-1137.
• [5]Stein LD.Human genome:end of the beginning[J].Nature,2004(7011):915-916.
• [6]West WH,Tauer KW,Yannelli JR.Constant infusion of recombinant interleukin-2 in adoptive immunotherapy of advanced cancer[J].N Engl J Med,1987(316):898-905.
• [7]Chang HW,Roh JL,Jeong EJ,et al.Wnt signaling controls radiosensitivity via cyclooxygenase-2-mediated Ku expression in head and neck cancer[J].Int J Cancer,2008(1):100-107.
• [8]Vaira S,Friday E,Scott K,et al.Wnt/beta-catenin signaling pathway and thioredoxin-interacting protein(TXNIP)mediate the"glucose sensor"mechanism in metastatic breast cancer-derived cells MDA-MB-231[J].J Cell Physiol,2012(2):578-586.
• [9]Kendziorra E,Ahlborn K,Spitzner M,et al.Silencing of the Wnt transcription factor TCF4 sensitizes colorectal cancer cells to(chemo-)radiotherapy[J].Carcinogenesis,2011(12):1824-1831.

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