周长荣,温小军,刘磊
ZHOU Changrong,WEN Xiaojun,LIU Lei

• 1:贵州医科大学基础医学院生物学教研室

摘要(Abstract)：

目的:测定珊瑚姜精油和樟树叶精油对白纹伊蚊雌性成蚊的熏杀活性及熏杀蚊虫的作用机理。方法:用水蒸汽蒸馏法提取珊瑚姜块茎精油和樟树鲜叶精油,三角瓶法测定两种精油对白纹伊蚊雌性成蚊的熏杀活性,微板法检测两种精油对白纹伊蚊乙酰胆碱酯酶(ACh E)和非特异性酯酶(NSE)活性的影响。结果:珊瑚姜精油和樟树叶精油对白纹伊蚊24 h熏蒸LC50分别是80.208μg/cm3、20.709μg/cm3;在78.696μg/cm3和31.976μg/cm3剂量下,珊瑚姜精油和樟树叶精油对白纹伊蚊的KT50分别为35.759 min和6.341 min;两种植物精油对白纹伊蚊ACh E均有抑制作用,酶活性抑制率分别为29.97%和4.03%;两种精油对白纹伊蚊的NSE有促进作用,酶活性增长率分别为21.55%和18.62%。结论:樟树叶精油更适宜作为植物源灭蚊产品的基础材料。
Objective: To investigate the fumigating insecticidal activities of essential oils extracted from Zingiber corallinum Hance and fresh leaves of Cinnamomum camphora( L.) against the female adult mosquitoes of Aedes albopictus and the fumigant mechanism. Methods: Essential oils were extracted from roots of Zingber corallinum Hance and fresh leaves of Cinnamomum camphora by steam distillation. The fumigating activities of these essential oils against Aedes albopictus were measured by erlenmeyer flask method. The activation changes of acetylcholinesterase( Ach E) and non-specific esterase(NSE) of the female adults mosquitoes of Aedes albopictus fumigant treated with two essential oils were measured by the microplate assay. Results: The LC50 values of Zingber corallinum Hance and Cinnamomum camphora against Aedes albopictus at 24 h were 80. 208 μg / cm3、20. 709 μg /cm3 respectively.The KT50 values of Zingiber corallinum Hance and fresh leaves of Cinnamomum camphora( L.) against the female adults mosquitoes of Aedes albopictus at fumigating concentration 78. 696 and 31. 976μg / cm3 were 35. 759 and 6. 341 min respectively. The acetylcholinesterase of Aedes albopictus which was treated by Zingiber corallinum Hance and fresh leaves of Cinnamomum camphora( L.) was inhibited. The reduced rates were 29. 97% and 4. 03%. The esterase activity increased rates were 21. 55%and 18. 62%. Conclusion: The results indicate that extracted oil from Cinnamomum camphora( L.)is effective potential mosquitocide.

关键词(KeyWords)： 伊蚊属;植物精油类;熏杀活性;乙酰胆碱酯酶;非特异性酯酶
Aedes albopictus;plant essential oils;fumigating insecticidal activity;acetylcholinesterase;non-speci esterase

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅与贵州医科大学联合基金[黔科合LG字(2012)15号]

作者(Author): 周长荣,温小军,刘磊
ZHOU Changrong,WEN Xiaojun,LIU Lei

DOI: 10.19367/j.cnki.1000-2707.2016.02.010

参考文献(References)：

• [1]中国国家质量技术监督.GB/T 17322.10一1998中华人民共和国国家标准一农药登记卫生用杀虫剂的室内药效评价一驱避剂[S].1998.
• [2]杨舒然,刘起勇.白纹伊蚊的全球分布及扩散趋势[J].中国媒介生物学及控制杂志,2013(1):1-4.
• [3]抉巧梅.五种柏科植物精油对蚊虫的生物活性[D].中南林业科技大学,2012.
• [4]Prajapati V,Tripathi AK,Aggarwal KK,et al.Insecticidal,repellent and opiposition-deterrent activity of selected essential oils against Anopheles stephensi,Aedes aegypti and Culex quinquefasciatus[J].Bioresour Technol,2005(16):1749-1757.
• [5]Ma WB,Feng JT,Jiang ZL,et al.Fumigant Activity of 6Selected Essential Oil Compounds and Combined Effect of Methyl Salicylate And Trans-Cinnamaldehyde Against Culex pipiens pallens[J].J Am Mosq Control Assoc,2014(3):199-203.
• [6]Ma WB,Feng JT,Jiang ZL,et al.Fumigant activity of eleven essential oil compounds and their selected binary mixtures against Culex pipiens pallens(Diptera:Culicidae)[J].Parasitol Res,2014(10):3631-3637.
• [7]郁凯,彭映辉,申鸽,等.三种植物精油对致倦库蚊成蚊的熏蒸活性及其混配增效作用[J].农药学学报,2014(2):132-137.
• [8]赵玉强,甄天民,李士根,等.微板法检测蚊虫体内乙酰胆碱酯酶活性研究[J].中国媒介生物学及控制杂志,1999(2):99-101.
• [9]李春晓,董言德,赵彤言.淡色库蚊对残杀威抗性与酯酶和乙酰胆碱酯酶的关系研究[J].中国媒介生物学及控制杂志,2007(1):9-11.
• [10]刘敏,何建国,抉巧梅,等.两种圆柏属植物精油对蚊虫的熏蒸活性[J].中国生物防治学报,2015(4):581-585.
• [11]曾冬琴.小蓬草植物精油对吸血蚊虫的驱杀活性及作用机理的研究[D].中南林业科技大学,2010.
• [12]张云,彭映辉,何建国,等.四种植物精油对蚊虫的熏杀活性及酯酶的影响[J].中国生物防治学报,2013(4):497-502.
• [13]杨频.5种植物精油对蚊虫的熏蒸毒杀作用及驱避效果的研究[D].陕西师范大学,2004.
• [14]彭映辉,张云,陈飞飞,等.岭南花椒果实精油精油成分的分析以及对两种蚊虫的毒杀活性[J].中南林业科技大学学报,2010(2):60-63.
• [15]张云,彭映辉,曾冬琴,等.竹叶花椒果实精油对两种蚊虫的毒杀活性研究[J].广西植物,2010(2):274-279.
• [16]王桂清,姬兰柱,张弘.辽细辛精油对淡色库蚊的杀伤作用[J].中国生物防治,2008(2):112-115.
• [17]杨频,潘沁红,马雅军.植物精油熏杀致倦库蚊对酯酶活性的影响[J].中华卫生杀虫药械,2005(4):235-237.
• [18]伍一军,冷欣夫.杀虫剂的神经毒理学研究进展[J].昆虫学报,2003(3):382-389.
• [19]Silvia B,Eduardo Z,Maria I P,et al.Activity of increased specific and non-specific esterases and glutathione transferases associated with resistance to permethrin in pediculus humanus capitis(phthiraptera:pediculidae)from Argentina[J].Parasitology Research,2010(2):415-421.

文章评论(Comment)：