康颖倩,金方,刘涛华,牟丽丽,吕倩,王颜颜,曾佳,胡晓霞
KANG Yingqian,JIN Fang,LIU Taohua,MOU Lili,LV Qian,WANG Yanyan,ZENG Jia,HU Xiaoxia

• 1:贵阳医学院微生物学教研室
• 2:贵阳医学院生物化学与分子生物学教研室
• 3:贵阳医学院生理教研室

摘要(Abstract)：

目的:研究放线菌菌株GYM39-2发酵液对临床常见病原性细菌及真菌的抗菌活性,同时观察温度、光照、pH值及紫外线等理化因素对发酵液稳定性的影响。方法:利用纸片扩散法测定放线菌株GYM39-2发酵液对临床病原菌的抗菌谱,观察改变温度、光照、pH及紫外线等理化因素时对发酵液的抗菌活性及稳定性的影响。结果:菌株GYM39-2对金黄色葡萄球菌、大肠埃希菌、乙型副伤寒沙门菌、白念珠菌及新生隐球菌均有抗菌活性,发酵液对温度、光照的改变及紫外线的照射均有较好的稳定性,pH在5~7之间发酵液抗菌活性最强。结论:菌株GYM39-2发酵液具有良好的抗菌活性及稳定性,能为相关研究提供菌株资源。
Objective: To study the antibacterial and anti-fungal activities of the fermentation broth from an actinomyocetes strain,GYM39- 2,and to verify the stability of the broth under different biochemical and physical conditions. Methods: Tests on bacterial and fungal susceptibility to the broth were carried out with Kirby-Bauey method,and the the broth stability under different temperatures,illuminations,pH,and ultraviolet conditions were observed. Results: The broth showed good antibacterial and anti-fungal ability against Staphylococcus aureus,Escherichia coli,Salmnella paratyphi B,Candida albicans and Cryptococcus neoformans. The changes of temperature,illumination and ultraviolet did not affect the broth's stability and the optimal pH range was 5 ~ 7. Conclusion: The fermentation broth of the actinomyocetes strain,GYM39- 2 has good antibacterial and anti-fungal ability. The results of this study provides new strain resource for novel antibiotics,enzymes and other biologically active substances,for both basic research and commercial application.

关键词(KeyWords)： 放线菌;发酵液;微生物敏感性试验;抗菌活性;稳定性
actinomyocetes;fermentation broth;microbial sensitivity test;antibacterial activity;stability

Abstract:

Keywords:

基金项目(Foundation): 国家自然基金地方项目(31060006,31260029);; 贵州省社会发展科技攻关项目[黔科合SY字(2011)3017号];; 贵州省卫生厅科技项目(gzwkj2010-1-025);; 贵阳市科技局社会发展与民生计划[筑科合同(2011)10316号]

作者(Author): 康颖倩,金方,刘涛华,牟丽丽,吕倩,王颜颜,曾佳,胡晓霞
KANG Yingqian,JIN Fang,LIU Taohua,MOU Lili,LV Qian,WANG Yanyan,ZENG Jia,HU Xiaoxia

DOI: 10.19367/j.cnki.1000-2707.2014.05.004

参考文献(References)：

• [1]李凡,刘晶星.医学微生物学[M].北京:人民卫生出版社,2008:184-186.
• [2]Bérdy J.Bioactive Microbial Metabolites[J].Antibiot,2005(1):1-26.
• [3]Arcamone F,Cassinelli G,Fantini G,et al.14-hydroxydaunomycina new antitumor antibiotic from S.Peucetius var caesius.Reprinted from Biotechnology and Bioengineering[J].Biotechnol Bioeng,2000(6):704-713.
• [4]Malla S,Niraula NP,Singh B,et al.Limitations in doxorubicin production from Streptomyces peucetius[J].Microbiol Res,2010(5):427-435.
• [5]许凤春,董美玉,张玲玲,等.放线菌G1—8菌株发酵液抗菌谱及稳定性的研究[J].生物技术,2008(4):81-83.
• [6]孙建龙,于基成,刘志恒,等.一株拮抗番茄溃疡病菌的海洋放线菌的分类鉴定及活性测定[J].吉林农业大学学报,2012(4):391-394.
• [7]魏松红,姚翠霞,张吉良,等.放线菌FJ12菌株发酵产物理化性质的测定及提取除草活性物质的条件优化[J].沈阳农业大学学报,2013(2):153-159.
• [8]贺建武,刘祝祥,龙华,等.波赛链霉菌JMC06001抑菌活性物质的特性研究[J].微生物学杂志,2011(1):10-14.
• [9]李艳利,黎晶晶,马中良.链霉菌Streptomyces.sp NJ0510的分离、鉴定及其抑菌活性的研究[J].生物技术,2007(4):69-71.
• [10]冯俊涛,张锦恬,韩立荣,等.放线菌HJ122菌株发酵液抑菌谱及稳定性的研究[J].西北农业学报,2009(6):280-284.
• [11]莫非,黄志卓,渠巍,等.323例院内感染革兰阳性球菌耐药性分析[J].贵阳医学院学报,2010(3):288-290.
• [12]周旋,陈晓霞,李雅君,等.2009-2012年金黄色葡萄球菌医院感染分布及耐药趋势[J].贵阳医学院学报,2013(3):327-331.
• [13]苏金石,谢峥,杨宗芳.抗生素耐药性的产生及预防[J].华西药学杂志,2003(4):279-280.
• [14]Lazzarini A,Cavaletti L,Toppo G,et al.Rare genera of actinomycetes as potential producers of new antibiotics[J].Antonie van Leeuwenhoek,2000(4):399-405.
• [15]杨晓楠,李杨,苗建强.拮抗放线菌T111菌株鉴定、发酵液理化性质测定及发酵条件优化[J].应用与环境生物学报,2011(4):542-547.

文章评论(Comment)：