贵州医科大学学报

2020, v.45;No.232(01) 23-28

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EGF对三阴乳腺癌细胞迁移和侵袭的影响及CANP-YAP通路的介导作用
Effect of EGF on Migration and Invasion of Triple Negative Breast Cancer Cells and Mediation of CANP-YAP Pathway

张莹;金爱;王旭东;
ZHANG Ying;JIN Ai;WANG Xudong;Department of Physiology, School of Basic Medical Sciences, Guizhou Medcial University;

摘要(Abstract):

目的:探讨上皮生长因子(EGF)对人三阴乳腺癌(TNBC)细胞迁移、侵袭能力的影响及CANP-YAP的介导作用。方法:以人源乳腺癌细胞系MDA-MB-231和468细胞为模型细胞,实验分为磷酸盐缓冲液(PBS)组、EGF组、EGF联合钙蛋白酶抑制剂(Cal)组(EGF联合Cal)及EGF联合钙蛋白酶抑制剂Ⅲ(CIⅢ)组(EGF联合CIⅢ),采用伤口愈合实验检测细胞迁移能力、Transwell检测细胞侵袭能力及Western blot法检测YAP蛋白在细胞中的表达水平。结果:伤口愈合实验和Transwell实验结果显示,EGF组MDA-MB-231、468细胞的迁移率和侵袭率均高于PBS组(P<0.05或P<0.01),EGF联合Cal组和EGF联合CIⅢ组中MDA-MB-231、468细胞的迁移率和侵袭率均低于EGF组(P<0.05);Western blot实验结果显示,EGF组MDA-MB-231细胞和MDA-MB-468细胞中YAP蛋白表达均高于PBS组(P<0.05或P<0.01);EGF联合Cal组和EGF联合CIⅢ组中MDA-MB-231、468细胞YAP蛋白表达均低于EGF组(P<0.05或P<0.01)。结论:EGF具有诱导TNBC细胞MDA-MB-231、468细胞迁移和侵袭,Cal和CIⅢ可以阻断EGF的上述生物学效应,EGF可能通过CANP-YAP信号通路促进肿瘤细胞的恶性生物学行为。
Objective: To investigate the effect of epithelial growth factor(EGF) on the migration and invasion of human triple negative breast cancer(TNBC) cells and the mediation of CANP-YAP pathway. Methods: Human breast cancer cell lines MDA-MB-231 and MDA-MB-468 were used as model cells, which were divided into the phosphate buffer(PBS) group, EGF group, EGF combined with calpeptin(Cal) group, and EGF combined with calpain inhibitor(CI) III group. The migration ability of the cells was measured by wound healing test. Transwell was used to detect the invasive ability of the cells, and Western blot was used to detect the expression level of target protein in cells. Results: The migration rate of MDA-MB-231 and-468 cells in EGF group was higher than that in PBS group(P<0.05), and the invasion rate of the two kinds of cells in EGF group was higher than that in PBS group(P<0.05 or P<0.01); The YAP protein expression of the two kinds of cells in EGF group was higher than that in PBS group(P<0.05 or P<0.01); The migration rate of the two kinds of cells in EGF combined with Cal group and EGF combined with CI III group was lower than that in EGF group(P<0.05).The invasion rate of the two kinds of cells in EGF combined Cal group and EGF combined CI III group was lower than that in EGF group(P<0.05), and the expression of YAP protein in EGF combined Cal group and EGF combined CI III group was higher than that in EGF group(P<0.05 or P<0.01). Conclusion: EGF has the ability to induce migration and invasion of model cells. Cal and CI III can block the above biological effects induced by EGF, suggesting that EGF can promote malignant biological behavior of cancer cells through CANP-YAP signaling pathway.

关键词(KeyWords): 乳腺肿瘤;细胞迁移;上皮生长因子;钙蛋白酶;YES相关蛋白
triple negative breast cancer;cell migration;epithelial growth factor;calpain;YES associated protein

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31660345,31360252)

作者(Author): 张莹;金爱;王旭东;
ZHANG Ying;JIN Ai;WANG Xudong;Department of Physiology, School of Basic Medical Sciences, Guizhou Medcial University;

Email:

DOI: 10.19367/j.cnki.1000-2707.2020.01.005

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