王露;何常;胡斌;李珀;
WANG Lu;HE Chang;HU Bin;LI Po;Teaching Division of Pathology,Guizhou Medical University;Department of Pathology,the Affiliated Hospital of Guizhou Medical University;

• 1:贵州医科大学病理学教研室
• 2:贵州医科大学附院病理科

摘要(Abstract)：

目的:探讨Nek8在结直肠癌组织中的表达及意义。方法:应用免疫组化SP法检测配对的40例结直肠癌患者的癌组织和结直肠正常黏膜组织、18例结直肠黏膜慢性炎症、18例结直肠管状腺瘤和50例结直肠管状腺癌(II级)中的Nek8蛋白表达水平,采用spearman分析Nek8表达水平与结直肠癌组织、正常黏膜组织和慢性炎症组织的相关性,分析Nek8表达水平与结直肠癌临床病理参数的关系。结果:在40例配对的标本中,结直肠癌组织中Nek8的表达阳性率(62.5%)高于正常黏膜组织(15.0%)(P<0.05);结直肠管状腺瘤和管状腺癌组织中Nek8的表达阳性率(83.3%,84.0%)明显高于结直肠黏膜慢性炎症组织(22.2%)(P<0.05),Nek8在结直肠黏膜慢性炎症、管状腺瘤及管状腺癌组织中的阳性表达存在正相关关系(rs=0.442,P<0.001);结肠癌组织中Nek8的表达水平与患者性别、年龄、5年生存率及癌组织的浸润深度、分期、是否发生淋巴转移无关(P>0.05)。结论:Nek8在结直肠癌组织中表达上调,可能参与了结直肠癌的发生发展。
Objective:To investigate the expression and significance of Nek8 in colorectal carcinoma tissues. Methods:Adopting IHC SP method tested and paired 40 cases of colorectal carcinoma tissues and healthy colorectal mucosa tissues,18 cases colorectal chronic inflammation,18 cases of colorectal tubular adenoma,and Nek8 protein expression level of 50 cases of colorectal tubular adenocarcinoma( Level II); adopting Spearman to analyze expression level of Nek8 and correlation among colorectal carcinoma tissues,healthy colorectal mucosa tissues and chronic inflammation tissues. Results:Positive expression rates of Nek8 protein in colorectal carcinoma( 62. 5%) was higher than those in healthy colorectal mucosa( 15. 0%,P < 0. 05) in 40 paired samples; positive expression rates of Nek8 protein increased in colorectal tubular adenoma and colorectal tubular adenocarcinoma( 83. 3%,84. 0%) were obviously higher than those of colorectal mucosal chronic inflammatory( 22. 2%,P < 0. 05). There was positive correlation among Nek8 expression in chronic inflammation,colorectal tubular adenoma and colorectal tubular adenocarcinoma( rs= 0. 442,P < 0. 001); there was no correlation between Nek8 protein expression level in colorectal carcinoma tissue and sex,age,depth of invasion,tumor staging,five year survival rate,metastasis of the patient( P > 0. 05). Conclusion:The expression of Nek8 is up-regulated in colorectal carcinoma tissues,which may be involved in tumorigenesis and development of colorectal carcinoma.

关键词(KeyWords)： 免疫组织化学;Nek8;结直肠肿瘤;腺瘤息肉病,结肠;慢性炎症
immunohistochemistry;Nek8;colorectal neoplasms;adenomatous polyposis coli;chronic inflammation

Abstract:

Keywords:

基金项目(Foundation): 贵州省科技厅基金(E2014-4)

作者(Authors): 王露;何常;胡斌;李珀;
WANG Lu;HE Chang;HU Bin;LI Po;Teaching Division of Pathology,Guizhou Medical University;Department of Pathology,the Affiliated Hospital of Guizhou Medical University;

DOI: 10.19367/j.cnki.1000-2707.2017.03.024

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