陈永香,文芳,吴金健,吴章颖,訾聃
CHEN Yongxiang,WEN Fang,WU Jinjian,WU Zhangying,ZI Dan

• 1:贵州医科大学临床医学院
• 2:贵州医科大学附院临床研究中心
• 3:贵州医科大学附院妇产科

摘要(Abstract)：

目的:探讨上皮性卵巢癌(EOC)组织中趋化因子受体4(CXCR4)、肿瘤干细胞分子标记物(CD133、CD44)的表达与临床病理之间的关系。方法:选择88例EOC患者卵巢肿瘤组织标本作为EOC组,同期80例卵巢良性上皮性肿瘤患者组织标本作为对照组,采用免疫组织化学法检测两组组织中CXCR4、CD44及CD133的表达,分析CXCR4、CD44、CD133表达与EOC患者临床病理特征的关系。结果:CXCR4主要表达于细胞质中,而CD44、CD133主要表达于细胞膜上,EOC组3项指标的表达显著高于对照组,差异有统计学意义(P<0.05);CXCR4及CD44表达均与临床分期、腹水中找到恶性细胞、淋巴结转移、大网膜转移有关(P<0.05),CD133表达与临床分期、淋巴结转移、大网膜转移有关(P<0.05),但3项指标均与年龄及病理类型无关(P>0.05)。结论:CXCR4、CD44及CD133表达可能与上皮性卵巢癌侵袭有关。
Objective: To investigate the relationship between the expression of chemokine receptor 4(CXCR4), tumor stem cell molecular markers(CD44, CD133) and clinicopathological features of epithelial ovarian carcinoma(EOC). Methods:A total of 88 specimens of ovarian tumors from patients with EOC were selected as EOC group and 80 specimens from patients with benign epithelial ovarian tumors were selected as control group.Immunohistochemical method was used to detect CXCR4, CD44 andCD133 expression, and the relationship between CXCR4, CD44 and CD133 expression and clinicopathological features of patients was analyzed. Results:CXCR4 was mainly expressed in the cytoplasm, while CD44 and CD133 were mainly expressed in the cell membrane. The expression levels of CXCR4, CD44 and CD133 in EOC group was higher than those in the control group, the differences were statistically significant(P<0.05).The expression of CXCR4 and CD44 was correlated with clinical stage, malignant cells found in ascites, lymph node metastasis and omental metastasis(P<0.05), and the expression of CD133 was correlated with clinical stage, lymph node metastasis and omental metastasis(P<0.05).However, there was no correlation between the three indexes and age or pathological type(P>0.05).Conclusion:The CXCR4, CD44, CD133 also play a role in the invasion and metastasis of epithelial ovarian cancer.

关键词(KeyWords)： 趋化因子受体4;上皮性卵巢癌;免疫组织化学;CD44;CD133
chemokine receptor 4;epithelial ovarian cancer;immunohistochemical;CD44;CD133

Abstract:

Keywords:

基金项目(Foundation): 贵州省教育厅科技拔尖人才支持项目[贵州省普通高等学校科技拔尖人才支持计划,黔教合KY字(2016)072];; 贵阳市科技计划攻关项目[筑科合同(2018)1-91];; 贵州省贵阳市科技局国基培育项目(筑科合同GY2015-40)

作者(Author): 陈永香,文芳,吴金健,吴章颖,訾聃
CHEN Yongxiang,WEN Fang,WU Jinjian,WU Zhangying,ZI Dan

DOI: 10.19367/j.cnki.1000-2707.2019.09.019

参考文献(References)：

• [1] BRAY F,FERLAY J,SOERIOMATARAM I,et al.Global cancer statistics 2018:globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries [J].CA:ACancer Journal for Clinicians,2018,68(6):394-424.
• [2] 曹泽毅.中华妇产科学[M].3版.人民卫生出版,2014.
• [3] LIEBER S,REINARTZ S,RAIFER H,et al.Prognosis of ovarian cancer is associated with effector memory CD8+ T cell accumulation in ascites,CXCL9 levels and activation-triggered signal transduction in T cells [J].Oncoimmunology,2018,7(5):e1424672.
• [4] VOELKER R.Pursuing an effective ovarian cancer vaccine [J].JAMA,2018,320(9):858-860.
• [5] YIN Y,LIU L,ZHAO Z,et al.Simvastatin inhibits sonic hedgehog signaling and stemness features of pancreatic cancer [J].Cancer Letters,2018,426:14-24.
• [6] CAO H,LI W,ZHOU Y,et al.Oroxylin a inhibits the protection of bone marrow microenvironment on CML cells through CXCL12/CXCR4/P-gpsignaling pathway[J].Frontiers in Oncology,2019,9:188.
• [7] FU Q,LI W,LI S,et al.CD44 facilitates adherence of Streptococcus equi subsp.zooepidemicus to LA-4 cells [J].Microbial Pathogenesis,2019,128:250-253.
• [8] OTTEVANGER P B.Ovarian cancer stem cells more questions than answers [J].Seminars in Cancer Biology,2017,44:67-71.
• [9] FIGUERAS A,ALSINA-SANCHIS E,LAHIGUERA á,et al.A role for CXCR4 in peritoneal and hematogenous ovarian cancer dissemination [J].Molecular Cancer Therapeutics,2018,17(2):532-543.
• [10] ERHART F,BLAUENSTINER B,ZIRKOVITS G,et al.Gliomasphere marker combinatorics:multidimensional flow cytometry detects CD44+/CD133+/ITGA6+/CD36+ signature [J].Journal of Cellular and Molecular Medicine,2019,23(1):281-292.
• [11] OLDHAM W M,VANEPS N,PREININGER A M,et al.Mechanism of the receptor-catalyzed activation of heterotrimeric G proteins [J].Nature Structural &Molecular Biology,2006,13(9):772-777.
• [12] PAWIG L,KLASEN C,WEBER C,et al.Diversity and inter-connections in the CXCR4 chemokine receptor/ligand family:molecular perspectives [J].Frontiers in Immunology,2015,6:429.
• [13] TONG W W,ZHANG C,HONG T,et al.Silibinin alleviates inflammation and induces apoptosis in human rheumatoid arthritis fibroblast-like synoviocytes and has a therapeutic effect on arthritis in rats [J].Scientific Reports,2018,8(1):3241.
• [14] ZIEGLER M E,HATCH M M,WU N,et al.MTORC2 mediates CXCL12-induced angiogenesis [J].Angiogenesis,2016,19(3):359-371.
• [15] ZHANG T,KAWAGUCHI N,YOSHIHARA K,et al.Silibinin efficacy in a rat model of pulmonary arterial hypertension using monocrotaline and chronic hypoxia[J].Respir Res,2019,20(1):79.
• [16] WANG X,CAO Y,ZHANG S,et al.Stem cell autocrine CXCL12/CXCR4 stimulates invasion and metastasis of esophageal cancer [J].Oncotarget,2017,8(22):36149-36160.
• [17] WENG C C,HAWSE J R,SUBRAMNIAN M,et al.KLF10 loss in the pancreas provokes activation of SDF-1 and induces distant metastases of pancreatic ductal adenocarcinoma in the KrasG12D p53flox/flox model [J].Oncogene,2017,36(39):5532-5543.
• [18] SUN Y,YOSHIDA T,OKABE M,et al.Isolation of stem-like cancer cells in primary endometrial cancer using cell surface markers CD133 and CXCR4 [J].Translational Oncology,2017,10(6):976-987.
• [19] KIMURA T,WANG L,TABU K,et al.Identification and analysis of CXCR4-positive synovial sarcoma-initiating cells [J].Oncogene,2016,35(30):3932-3943.

文章评论(Comment)：