宁晓丽,王炎,余跃
NING Xiaoli,WANG Yan,YU Yue

• 1:安徽医科大学附属巢湖医院麻醉与疼痛科

摘要(Abstract)：

目的 分析白藜芦醇通过调控Wnt/β-catenin信号通路抑制胶质瘤细胞的机制。方法 采用0（对照组）、25（低剂量组）、50（中剂量组）以及100μmol/L（高剂量组）白藜芦醇对胶质瘤细胞U251进行处理，比较各组细胞培养24、48以及72 h时的细胞活力、侵袭个数、迁移个数、凋亡率，比较各组细胞培养72 h时的Wnt3a、β-catenin蛋白表达及mRNA水平。结果 与对照组相比，各白藜芦醇培养组细胞培养24、48以及72 h时细胞活力均降低，细胞侵袭、迁移个数均减少，细胞凋亡率均升高，且各白藜芦醇培养组细胞活力随培养时间的延长而逐渐降低，细胞侵袭、迁移个数均随培养时间的延长而增加，细胞凋亡率均随培养时间的延长而增加（P<0.05）；各培养时间下，白藜芦醇培养低剂量组的细胞活力均最高、细胞侵袭和迁移个数均最多、细胞凋亡率均最低，而高剂量组细胞活力最低、细胞侵袭和迁移个数均最少、细胞凋亡率均最高，差异均有统计学意义（P<0.025）；培养72 h时，与对照组比较，各白藜芦醇培养组细胞Wnt3a、β-catenin蛋白表达及mRNA水平均降低，且低剂量组的细胞Wnt3a、β-catenin蛋白表达及mRNA水平最高，高剂量组最低，差异有统计学意义（P<0.05）。结论 白藜芦醇可能通过抑制Wnt/β-catenin信号通路抑制胶质瘤细胞活性，减少细胞侵袭和迁移，促进细胞凋亡。
Objective To explore the mechanism of resveratrol in inhibiting glioma cells by regulating the Wnt/β-catenin signaling pathway.Methods Glioma cells(U251) were treated with resveratrol and divided into at the following groups based on the concentrations of resveratrol: 0 μmol/L(control group), 25 μmol/L(low dose group), 50 μmol/L( medium dose group) and 100 μmol/L(high dose group). Cell viability, invading cell numbers, migrating cell numbers and apoptosis rates were compared at 24 h, 48 h, and 72 h among above groups. Both protein and mRNA expression levels of Wnt3a and β-catenin were compared at 72 h after the treatment among these groups.Results When compared to control group at 24 h, 48 h, and 72 h after the treatment, cell viability was decreased,and the numbers of invading cells and migrating cells were reduced in low-, medium-and high-dose groups, while apoptotic rates were increased. In addition, cell viability was decreased in a timedependent manner, while the numbers of invasive cells and migrating cells as well as apoptotic rates were increased over the time(P< 0. 05). At 24 h,48 h, and 72 h, low dose group had the highest cell viability, the highest number of invading cells and migrating cells and the lowest apoptotic rate among resveratrol-treated groups, contrary to high dose group. The difference was significant(P< 0. 025).When compared to control group at 72 h after the treatment, both protein and mRNA levels of Wnt3a and β-catenin were reduced in low-, medium-and high-dose groups. Low-dose group had the highest protein and mRNA levels of Wnt3a and β-catenin among resveratrol-treated groups, contrary to high dose group. The difference was significant(P< 0. 025).Conclusion Resveratrol can impair glioma cell viability by inhibiting the Wnt/β-catenin signaling pathway, reducing cell invasion, and migration and promoting cellular apoptosis.

关键词(KeyWords)： 胶质瘤;白藜芦醇通过;Wnt/β-catenin通路;活性;侵袭;迁移;凋亡
glioma;resveratrol;Wnt/β-catenin pathway;viability;invasion;migration;apoptosis

Abstract:

Keywords:

基金项目(Foundation): 安徽省科学技术厅重点研究和开发计划项目（1804ho8020272）

作者(Author): 宁晓丽,王炎,余跃
NING Xiaoli,WANG Yan,YU Yue

DOI: 10.19367/j.cnki.2096-8388.2022.04.008

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